Acc Deaminase Plant Grows Bacteria Screening And Its Feasibility In Petroleum Contaminated Soil Restoration

With the increasing of petroleum productions and consumption, environment pollution is gradually severe. Oil-contaminated soil is one of the environmental problems resulting from petroleum production, and one desirably solved. The traditional physical and chemical methods need a high cost, also can cause additional pollution. Therefore, the removal of oil by utilizing the plants is one of effective technologies for the remediation for oil-contaminated soil. Compared to the traditional physical and chemical methods. Phytoremediation has being developed recently in the field of environmental protection. Due to its advantages in a low cost, free additional pollution and moderate conditions, the method has been studied and developed extensively in the world.In this paper, we carried out Phytoremediation technology to the petroleum contaminated soil and provided theoretical guidance. The study of Infrared spectrophotometry, results showed that the purity of carbon tetrachloride has certain effect on the accuracy of the measurement results of the oil content of contaminated soil. At the same time, The determination results of gravimetric and infrared method was informed that the oil content is higher by the gravimetric method than the result by infrared method. Experiment of the isolation of plant growth-promoting bacteria (PGPR) from oil contaiminated soil of Sheng li and Da gang Oil field, was carried out with the method of enrichment screening as well as that ACC (1-amino cyclopropane-1-carboxylic acid) wasused as the sole nitrogen source. Twelve strains PGPR were isolated. Through the determination of production of ACC deaminase activity, siderophore and phosphate solubilization capacity, five strains PGPR were strains of the advantage. In this paper, the resistance of five strains PGPR on salt and pH were studied, the results show that five strains have a certain resistance, which the resistance of strains D5A is obvious. Through the germination experiments, we studied promoting effect of the five strains on tall fescue under different salt conditions. The results showed that the strains D5A could significantly improve the growth of tall fescue. Finally, the strains was identified as Klebsiella pneumoniae. As its physiological, biochemical characteristics and16S rDNA sequence were analysed.The biological toxicity of strains D5A and strains S1C was tested. On the basis, the pot experiment carried out to study the influence of efficiency of tall fescue remediation petroleum-contaminated soil by inoculated strains D5A. Inhibited the growth of tall fescue to a certain extent by the contaminated soil, coupled with the complexity of the contaminated soil itself, the result was not clearly reflected the strains D5A promoting effect. The promoting effect of strains D5A remains to be further research.