Japan's Triangle Planaria (dugesia Japonica) The Comparison Of Lead Stress Mitochondrial Proteome Research

In all kinds of environmental pollution, the pollution in water is especial serious, whose harm is the biggest and most widespread. Heavy metal element, for example lead, could be released to the environment by many kinds of way, like mining, smelting, coal burning, automobile exhaust discharging, galvanization industry and so on. In addition, the subsidence of atmospheric dust particle and the washing of rain water to ground, all may cause it to enter in the rivers and streams lake, causes the water body pollution, affects aquatic biological and humanity’s activity. Therefor to develop research about aquatic biological pollution is urgent.The lead could reduce the content of mitochondrion, so it could affect the energy metabolism in organism. Disturbance of mitochondrial energy metabolism is the common result of multiple complex factors, especially when the body suffered from burn, trauma, stress or heavy metal. There is little knowledge on the entire mitochondria injury and compensation response during the development of energy metabolism disturbance, so few methods was found effectively in alleviating the disturbance of mitochondrial energy metabolism. Comparative proteomics gives us a global description of the differential expression of organizational mitochondria proteins in the development of energy metabolism disturbance under stress of Pb, and shed light on the exploration of upstream molecular mechanism, therefore, effective methods alleviating the post-burn energy metabolism disturbance may be found.In this study, comparative proteomics was used to reveal the differential expression of mitochondria proteins in Dugesia Japonica under stree of Pb, followed by evaluating the effect induced by changed expression of key proteins. Differential proteins of mitochondria were detected to explore the molecular mechanism of damage caused by Pb. The main conclusion as follows:1. The purity and integrity of purified myocardial mitochondria isolated by Nycodenzdensity gradient centrifugation are proved quite well through electron microscope and westernblot analysis.2. Well focused and distinct2-DE maps with good reproducibility were obtained. All of the maps were analysed with ImageMaster TM software, a total of19differential expression proteins was successfully identified by MALDI-TOF-MS.3. Eight of the ninteen differential expression proteins show a downregulated expression in Pb treatment groups, they are voltage-dependent anion-selective channel, predicted protein, lysozyme precursor (EC3.2.1.17), beta actin-like1protein, alpha-tubulin, keratin,type Ⅱcytoskeletal1, keratin, type I cytoskeletal10. The decreased expression may result in the disturbance of ATP synthesis, Protein synthesis and degradation, Cell structure and motility and apoptosis induction. eleven of the ninteen differential expression proteins show a upregulated expression in Pb treatment groups, they are Cytochrome c oxidase polypeptide Va, mitochondrial precursor, putative, PREDICTED:hypothetical protein LOC100706456, liver regeneration-related protein2, PREDICTED:RING finger protein213-like, beta-tubulin, keratin9(epidermolytic palmoplantar keratoderma), Chain B, Refined1.8Angstroms Resolution Crystal Structure Of Porcine Epsilon-Trypsin. They may be the compensation effect to resist the injury.