Tetrabromo Bisphenol - A With The Interaction Of Dna And Its Toxicity Studies

Many of the chemical pollutants in environment will lead to damage of DNA molecules by taking part in the process of metabolism. The DNA adduct is the most common form of chemical injury, and it reflects the reaction of pollutants with DNA and can be used to evaluate the potential carcinogenicity.Due to the extensive use of TBBPA, TBBP-A has been found in the air, soil, water sediments, seabirds and fish, and even human blood, breast milk and other environmental media and biological samples. Meanwhile, because1-Aminopyrene and DNA can form adduct has been recognized, so we want to study the DNA adduct and DNA damage by choosing1-amino pyrene and tetrabromobisphenol-A as the objects, pUC18plasmid DNA as experimental material, using DNA gel electrophoresis and absorption spectrum method. In the research, we have studied the interactions of1-AP, TBBP-A and DNA in the four systems respectively. The experimental work mainly includes four parts:(1) The Interaction of1-AP and TBBP-A with DNA: The DNA gel electrophoresis and UV spectra shows that no adduct were found when1-AP was mixed with DNA. The adduct of TBBP-A and DNA was also not found.(2) Interaction of1-AP and TBBP-A with DNA irradiated with xenon light: When the1-AP and the DNA placed in mixed and then under the lighting conditions, the electrophoresis image of the sample prove the injury trend and UV overlay also prove that they are bouded. Study TBBP-A mixed with DNA under the same conditions of light, the electrophoresis image has also undergone significant injury trends and changes along with the UV spectra, the conclusion is that TBBP-A and DNA are interacted.(3) Interaction of1-AP and TBBP-A with DNA in the Fenton production of free radicals system:1-AP and DNA electrophoresis image of the mixed in Fenton production of hydroxyl radicals system prove a damage trends, and change1-AP, DNA and Fe2+response rate, then DNA damage results will change also.when the dosage of the Fe2+reach300μL its damage reach half, when the dosage increased to500μL, the DNA is completly injurird; And change the TBBP-A, DNA with Fe2+response rate for the same reason can be drawn, then DNA damage results change, and TBBP-A on DNA damage as Fe2+amount of the increase was linear related.(4) Interaction of1-AP and TBBP-A with DNA in the presence of S9liver microsomal enzyme:in simulated in vivo metabolic system S9liver microsomal enzyme mixture, proving that1-AP and DNA has been reacted. Change the1-AP (TBBP-A), the proportion of the reaction of DNA with S9mixture, get the poison on DNA damage dose changes. With the S9to join to an increase and the amount of DNA damage proved quantitative linearly related. When compare with the results of1-AP with DNA in the S9liver microsomal enzyme system simulated in vivo experiments, prove that TBBP-A with DNA has been reacted also.