The Preparation Of New Fluorescent Derivative Reagent And Its Application In High Performance Liquid Chromatography (hplc)

As an important analytical method, High performance liquid chromatography is one of thefastest growing and most widely used areas in analytical chemistry. Because of its powerfulseparation and analysis capabilities, it’s applied to chemical and biochemical analysis, commonlyused in medicine, chemical, environmental, life sciences, and the food industry,the ingredients inthe mixture separation, composition qualitative and quantitative analysis applicable to theanalysis of large molecular weight of different polarity, high boiling point of less volatile organiccompounds. However, due to the nature and low content restrictions of the measuredcomponents, not all compounds can be direct use in liquid chromatography analysis. In recentyears, in order to improve the detection sensitivity, fluorescence detection method is rapiddevelopment, to mark the weak fluorescence and non-fluorescent substance labeled reagentshave also developed and put into use.This paper is mainly based total synthesis of a newfluorescent labeling reagent, and its derivative conditions for the chromatographic separationconditions, and optimization methods used in the actual samples.The specific work is as follows:Chapter One: The basics of high performance liquid chromatography；The application profilesof a fluorescent labeling reagent in the high performance liquid chromatography；Introduced theprinciple of fluorescence and fluorescent labelingChapter Two: Highly sensitive fluorescent labeling reagent4-(9-acridine ketone)-methylbenzoyl hydrazine synthesis, structural characterization.Chapter Three:4-(9-acridone ketone)-methyl benzoyl hydrazine as fluorescent labelingreagent for fatty acids by high performance liquid chromatography-mass spectrometry. Theoptimization of the conditions of the derivatization and chromatographic separation conditions,the establishment of a fatty acid column derivatization high performance liquid chromatographywith fluorescence detection, and the method is applied to the determination of free fatty acids inthe soil, testing the feasibility of the establishment of analytical methods.Chapter Four:4-(9-acridine ketone)–methyl benzoyl hydrazine for aliphatic aldehydes ofhigh performance liquid chromatography-mass spectrometry.Through the optimization of theconditions of derivatization and chromatographic conditions aliphatic aldehydes columnderivatization high performance liquid chromatography with fluorescence detection, and themethod for the determination of aliphatic aldehydes in the liquor, inspection of the analyticalmethod feasibility.Chapter Five: Fluorescent labeling reagent4-(9-acridine)-aminobenzoic acid to phenol by high performance liquid chromatography-mass spectrometry. The optimization of theconditions of the derivatization and chromatographic separation conditions, the establishment ofthe phenol column derivatization high performance liquid chromatography with fluorescencedetection, and the establishment of the method used in the detection of phenol in sewage,experimental results show that the established method has very large possibilities.