Three Experimental Rabbit Closed Group Of Microsatellite Dna Polymorphism Of Genetic Analysis

Three closed rabbit colonies including Japanese White Rabbits(JWR), Chinchilla Rabbits(CR), New Zealand Rabbits (NZWR) are generally used as conventional laboratory rabbits. Although they are considered as closed animals, their populations genetic stability and homogeneity are not clear because of the inappropriate conservation and hybridization. Given the current circumstances, we need to establish a scientific methods on laboratory rabbits genetic monitoring as soon as possible, including the establishment of genetic marker library of closed rabbit colonies, to identify the different strains of closed rabbit colonies and to monitor whether there is genetic differentiation among closed rabbit colonies, so that we may keep the entire population genetic stability and maintain the unity of consistency and polymorphism.Traditional genetic markers is the mark of the phenotype which is not a direct response to the nature of genetic changes, showing less polymorphisms and numbers. In this paper we choose microsatellite marker to analyse the population genetics of three closed rabbit colonies including Japanese White Rabbits(JWR), Chinchilla Rabbits(CR), New Zealand Rabbits (NZWR), screen the best genetic quality control markers of closed rabbit colonies.In this paper we selected10microsatellite loci from28microsatellite loci to analyse the population genetics of three closed rabbit colonies:Japanese White Rabbits(JWR), Chinchilla Rabbits(CR), New Zealand Rabbits (NZWR). The10microsatellite loci were selected for the test by Hardy-Weinberg Equilibrium (HWE), and the values of the allele frequency, the observed heterozygosity, the expected heterozygosity, the F-statistic and the genetic distance were counted. The results show: The microsatellite locus12L1E11in CR population, the loci INRACCDDV0087and INRACCDDV0203in NZWR population, and the loci Sat12and INRACCDDV0203in JWR population evidently deviate from the HWE (P<0.05), mostly resulting from heterozygote deficiency. The other microsatellite loci that Satl3, Sol44,6L1F10,7L1F1,12L4Al,and INRACCDDV0016in three populations all conform to the HWE. The average number of alleles is5.9, and the range of allele frequencies is from0to0.9060. The average observed heterozygosity is0.6204and the average expected heterozygosity is0.6178. The average PIC of the ten microsatellite loci is from0.430to0.834.The average Fst value among populations is0.0750. The lowest genetic distance observed is0.1223between JWR and CR, and the highest genetic distance observed is0.1934between CR and NZWR.The experients proved that the genetic structures of the three closed rabbit colonies show genetic stability and homogeneity. The10microsatellite loci have high genetic polymorphisms. There is evidently genetic differentiation among populations. The relationship between between JWR and CR is closed and The relationship between between CR and NZWR is far, which proved the relationship of three populations are not only influened by the factor of history, but also by the factors of geography and circumstance. We concluded that these ten microsatellite locus may be as the candidate of the genetic quality control markers in closed rabbit colonies.