Electric Field Induced By Aqueous Photoluminescence Of Research And Application In The Analysis Of Dna Sensor

Deoxyribonucleic acid (DNA) is a kind of important biological macromolecules and the carrier of genetic information organisms. The sequence of DNA research has an extremely important role in disease diagnosis, environmental science, life sciences, and so on. Since the human genome project, The research of DNA sequences has been a frontier subject in the field of biological science. In addition, it has formed a variety forms of DNA biological sensors gradually.The principle of DNA biosensor is classical. It transforms the hybridization event into light, electricity, sound signals which can be detected. DNA sensor usually composed of a sequence identification and a signal transformation devices. In general, the sequence identification device contains of probe DNA and other auxiliary content composition, which be able to identify specific sequence target and hybridize with it; The transducer changes the signal of hybridization event into the light, electricity, sound. According to the different signal conversion transducer, There are different types of DNA biosensors:The piezoelectric DNA sensor, Optical and Electrochemical DNA biological sensors.This thesis included a review and a research section. In the review, the composition of deoxyribonucleic acid and DNA biosensor’s research content were introduced. The DNA biosensor’s classification was summarized too. The research section contained two subunits. The mechanism of electric field induing luminescence phenomenons(EFIL) of aqueous solution was discussed. On the baseis of EFIL of aqueous solution, We established a modle of lable-free DNA sensing method. In addition, we tested the single base mismatch DNA sequences.1. The mechanism exploration of electric field induction luninescence phenomenon of aqueous solution.In the experimental process, We discovered the electric field induing luminescence phenomenons (EFIL) of aqueous solution, and discussed its mechanism. We had investigated the effect of electric field, magnetic field, anion and cation of electrolyte on the luminescencet EFIL int intensity. In addition, the electrical conductivity,17O MNR half peak width, fluorescence properties changes before and after electric field applying aqueous solution were investigated too. Finally, we discussed the mechanism of electric field induing luminescence phenomenons (EFIL) of aqueous solution. The result showed that the anion and cation directional moved at the electric field. It caused the hydrogen bonds breakage.The water clusters were decomposed into smaller water cluster structure. The hydrogen bond dissociation energy which could be transfered to the smaller water cluster units, and made them jump from the ground state to the excited state. When they back to the ground, the water cluster would accompany with the luminescence phenomenons.2. The luminescence phenomenon of electric field inducting aqueous solution used in lable-free DNA sensing method.The device for electric field induction aqueous solution luminescence was designed. First,50mM of Tris-HCl buffer was injected, and applyed pulse voltage. The strong luminescence signal can be observed under the electric field; At the same time, a certain concentration of DNA probe was injected to the buffer, The luminescence intensity was strongly inhibited. The luminescence intensity have a certain degree of recovery with the addition of complementary DNA. Under optimum conditions, We established a labe-free DNA sensing method. The decrement of luminescence intensity was proportional to the concentration of target DNA in the range of1.0x10-7～1.0x10-9mol/L, The detection limit was6.5x10-10mol/L.