Silica Carrier Forming And The Pvdf - The Sio < Sub > 2 < / Sub > Composite Ball Lipase Immobilized On Research

As a biological catalyst, lipase （EC3.1.1.3, triacylglycerol ester hydrolases） enables theirvarious applications: textile industry, food industry, pharmaceutical industry and fine chemistry.Lipase leads to the loss of the biological activity, and not easy to recycle. Immobilization ofenzyme has been a significant strategy for most industrial applications due to advantages ofproduct purification, continuous production, and biocatalyst recycling. What’s more,immobilization also improves enzyme stability while reduces energy consumption. So the keytechnique in the industrialization process of lipase catalysis is immobilization.The carrier is one of main factors in the technique of immobilization. Both the chemicalproperty and microscopic structure of carriers play an important role in the lipase loading,enzyme activity and activity recovery. So far, amount of carriers with different structures hasbeen developed. Among these carriers, silica materials as supports have been exploredextensively due to the ease in stable structure, environmental acceptability, and more resistant tomicrobial attacks. However, the weakness of silica materials is easy to reunite while difficult tomold. Due to the above disadvantages, silica powders as supports are unfavorable to apply toindustrial production filled in large reactor. To overcome the disadvantage of silica materials, theaim of this study was to mold carrier.Accordingly, the main work done and main results are summarized as followings:1. Selected columnar candida lipase as the research object and the silica particles ofdifferent hydrophily as immobilized carrier, prepared immobilized lipase by adsorption method,researched different hydrophilic carrier on the influence of immobilized lipase. The results showthat when carrier contact angle is about90-100°or so, the largest amount of CRL adsorptionappears.The quantity of the more hydrophilic or lyophobic carrier fell. When contact angle is94°, the adsorption quantity of silica is biggest which is160mg/g.It can be screened best carrier2. Polyvinylidene fluoride （PVDF）-silica（SiO₂） composite material containing SiO₂which was prepared by phase transition process was used as carrier to immobilize on CRL. It isfound that CRL immobilized on PVDF or PVDF-SiO₂composite material has a large loading. Asto PVDF-SiO₂composite material, with the increase of SiO₂amount, both the loading and therecovery efficiency of activity have increased. When the ratio of the composite material ofPVDF and SiO₂is1:1（quality ratio）, it has the maximal loading which could reach200mg/gand the maximal recovery efficiency of activity is50.98%, the PVDF-SiO₂composite material is the optimized support in the experiment. Compaired PVDF-SiO₂composite material with SiO₂,the former is superior to filtrate and has no pollution on substrate solution.