Studies On Biology Activity Of Rutin-metal Complexes

Rutin on the Sophora japonicaL attribute to the flavonoids,it has particular chemistry and medicine alive characters,gainning wide appliance in many field.Research and empolder（R&D） to it have important realism meaning.Study work has mainly five aspects:（1） This study orderly examines flow looks and choosing measure wavelength in the literature.Obtaining better separate degree and apex aitch of flow looks, 254nm as its measure wavelength by experimentation;（2） Choosing differt main strain and periodic metal salts complexe with rutin,properties of rutin-metal complexes;（3） Testing anti-bacterium ability for rutin and rutin-metal complexes;（4） Mensurate eliminating·OH ability for rutin and rutin-metal complexes and eliminate O₂^·- function;（5） Fringe studying for eliminating O₂^·- dynamics of rutin.Results of researching indicate:（1） Distilling of rutin adopts acid and alkali way,using carbinol purification,carbinol:water:ice acetic acid（40:59:1 v/v） as flow looks,measure wavelength is 254nm,linearity relation is all right（r=0.9992）,average reclaim ratio is 97.2%.（2） Rutin-metal complexes have been dealt with by using Wet break down meaning.adopting AAS determines content of rutin-metal complexes,relatively standard deviation less than 0.5%, mensurated result indication:this way can accidence confirm:metal with rutin have been compled.（3） Determine the nature experimentation adopts MH agar flat,accidence analysis anti-bacterium effection of rutin-rare dust complexes are greatly well than rutin-main group,rutin-assistant group and rutin;fix quantify examination adopts cuvette compare chaotic way,educing the MIC of rutin and rutin-metal complexs.（4） Anti-oxidation of rutin and rutin-metal complexes contrastion indicate:eliminating·OH and O₂^·- ability of rutin-metal complexs are greater than tutin,thereout experiment result indicates, rutin and rutin-metal complexs are a better elimination of freedom radicle.（5） This text study eliminate O₂^·- dynamics of rutin applys to UV and HPLC.First confirm oxidation of rutin’s absorbency quotietyε_1cm^254nm=2.49×10⁵;gained reaction progression in eliminating O₂^·- of rutin n=3.61;reaction activation energy Ea=11.76kJ/mol.