| Objective: The diabetic nephropathy (DN), as a common diabeticmicrovascular complications is one of the reasons caused the end stage renaldisease (ESRD). Therefore, it’s important to take actions in early stage tocurtail the deterioration of this disease. In clinic, the prescription ofYiQiYangYinHuoXue is effectively used to prevent the diabetic microvascularcomplications and protect renal function. The diabetic rat models wereestablished by intraperitoneal injection of streptozotocin (STZ). This researchwas observed the biochemical parameters, renal histomorphology changes,expression of PDGF-B in nephridial tissue of diabetic rats through interveningwith the prescription of YiQiYangYinHuoXue. Then explore the possiblemechanism of YiQiYangYinHuoXue’s prescription in preventing the earlystage DN.Methods: Fifty seven clean healthy male Wistar rats, with body weight200g±20g. After one week adaption,12rats were randomly selected as thenormal control group (N). The rest45rats were used for establishing model.According to the references, the rats were injected abdominal cavity with STZ(55mg/kg), which was dissolved into1%buffer solution of citric acid of STZ,the normal group were injected with the equal volume of1%citric acid-sodium citrate. After injection72hours, we measured the blood glucose andconsidered the blood glucose more than16.7mmol/L with increased waterintake and urine as the successful models.The diabetic rats were randomly divided into three groups, the modelgroup (M), the traditional Chinese medicine group (H), irbesartan group (W),each group was15rats. Then, the medicine was given to the rats as ten timesof human dosage, once a day. The Chinese medicine group of rats were given the YiQiYangYinHuoXue’s prescription with a dose of6.5mL/kg weight perday (equivalent to32.5g/kg herbs) by gavage, the irbesartan group were givenirbesartan with a dose of3.4mL/kg weight per day (equivalent to7.5mg/mLirbesartan) by gavage, the normal group and the model group were given theequal volume of saline. Each group was given the medicine for8weeks,during that time the rats’ eat and drink were free. At the end of the eighthweeks, we used the metabolic cages to collect24h urine of rats for the urinaryalbumin excretion rate (UAER). At the end of the experiment, the rats wereforbidden to eat for12h, and all rats were anesthetized by being injected inabdomen with2%napental. We got the blood from femoral artery forbiochemical test, the kidney was obtained for HE and PAS staining,immunohistochemical to observe the changes on pathomorphism of nephridialtissue and detect the expression of platelet derived growth factor B (PDGF-B)in nephridial tissue.Results:1Behavior observation of all the ratsThe rats in normal group, its mental station, appetite, reaction, urinevolume were normally, and body weight growth significantly; while the modelgroup showed apathetic, drinking and eating more than normal group, polyuria,loss weight obviously and body gradually thinner, fur color turned to darkyellow, slow reaction, some of the rats even appeared hemiplegica, abdominalswelling. Two treatment groups showed a similar situation, but theperformance was alleviated than the model group. During the experiment, onerat died in model group due to the hemiplegia and can’t eat. There were tworats dead in the traditional Chinese medicine group resulting from theimproper gavage, bites from each other and infection.2The blood glucose and cholesterol, triglyceride of the rats in each groupCompared with the normal group, the blood glucose in the model groupand two treatment groups was significant higher (P<0.05), compared with themodel group and irbesartan group, the blood glucose of the traditionalChinese medicine group was significant difference (P<0.05). There was no significant difference between the model group and irbesartan group (P>0.05).Compared with the normal group, the cholesterol and triglyceride of themodel group and two treatment groups increased significantly (P<0.05). Whilecompared with the model group and irbesartan group, the cholesterol andtriglyceride in traditional Chinese medicine group was significant lower(P<0.05), but those parameters in irbesartan group has no significantdifference with model group (P>0.05).3The body weight and kidney hypertrophy index (KW/BW) in each groupCompared with the normal group, the body weight of model group and thetwo treatment groups decreased significantly (P<0.05), and the kidneyhypertrophy index increased significantly (P<0.05). The body weight andkidney hypertrophy index of model group had significant difference from thetwo treatment groups (P<0.05).There was no difference between twotreatment groups (P>0.05).4The renal function and UAER in each groupThe blood urea nitrogen (BUN) and serum creatinine (SCr) of modelgroup were significant higher compared with normal group (P<0.05).Compared with the model group, the parameters in the two treatment groupswere decreased significantly (P<0.05), but no significant difference betweenthe two treatment groups (P>0.05).Compared with the normal group, the UAER of model group increasedsignificantly (P<0.05).The two treatment groups can significant reduce theUAER compared with model group (P<0.05), while between the twotreatment groups there was no significant difference (P>0.05).5Light microscopy observation of nephridial tissue sectionsIn normal group, the rats’ renal structure was complete, no increase inrenal glomerulus, cell texture was clearly, cell contorts were normal, nosignificant abnormalities in glomerulus capillary basement membrane andmesangial matrix. In model group, the renal glomerulus was increased,mesangial cell proliferation, and the basement membrane thickening. Thehistomorphology changes in the traditional Chinese medicine group and the irbesartan group had different levels of mitigation compared with the modelgroup.6Expression of platelet derived growth factor B in diabetic rats’ nephridialtissueThe expression of PDGF-B in model group and two treatment groups weresignificant higher than that in normal group (P<0.05). After the treatment,compared with the model group, expression of PDGF-B in two treatmentgroups were significant lower (P<0.05) in different levels. There was nodifference between the two treatment groups (P>0.05).Conclusions:1The prescription of YiQiYangYinHuoXue can protect the renal function,reduce the emission of urine microprotein, and delay the pathological progressin diabetic rats’ kidney. It was clearly suggested that this description had theprotective effect on diabetic kidney.2The mechanism of YiQiYangYinHuoXue’s prescription prevention andcurtail diabetic microvascular complication was probably related to lower theblood glucose, improve lipid metabolism and inhibit the expression ofplatelet derived growth factor B (PDGF-B). |
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