Effects Of Nedaplatin On The Radiosensitivity Of Human Nasopharyngeal Cancer

Objective:To study the radiosensitization of nedaplatin in nasopharyngeal carcinoma in vitro, as well as the possible mechanisms.Methods:Cell survival rate was assessed using CCK-8. The survival fraction of each group was investigated with colony formation assay after different treatments. The radiobiological parameters and sensitizing enhancement ratio (SER) were obtained from single-hit multi,-target models. Cell cycle distribution and apoptosis were detected by flow cytometry. Protein expressions of p-AKT and Bcl-2were measured with the Western-blot analysis.Results:Nedaplatin inhibited the proliferation of CNE-2cells in a time-and-concentration-dependent manner (p<0.05). We selected0.25mg/L and0.5mg/L nedaplatin for the subsequent experiments. The SERDO and SERDq were1.16and1.32respectively in the0.25mg/L nedaplatin treated group, while they were1.23and1.82at0.5mg/L nedaplatin treated group. After nedaplatin treatment for24h, the G2/M phase ratio of CNE-2cells were (4.28±1.20)%,(6.6±1.15)%and (9.2±0.65)%at0,0.25and0.5mg/L. The apoptotic ratio were (4.28±1.20)%,(6.6±1.15)%and (9.2±0.65)%respectively. The expressions of p-AKT and bcl-2were higher in the nedaplatin treatment groups than in the control group. Moreover, the protein level changed distinctly in the combination group. Conclusion:Nedaplatin could enhance radiosensitivity of CNE-2cell line, the mechanism could be related to modify the cell cycle and induced apoptosis.