The Effects Of L-form Variation On The Resistant Of Mycobacterium Tuberculosis And Antituberculosis Effect Of Aliazrin

Objective To establish the L-form induction model of Mycobacterium tuberculosis, fully evaluate the therapeutic effect of traditional Chinese medicine Aliazrin on the L-form M. tuberculosis, and provide an experimental basis for its vivo research and development of Chinese Drug Research.Methods①hrough combinations of three kinds of drugs at different concentrations and three kinds of Mycobacterium tuberculosis L-forms culture media, bacteria type of Mycobacterium tuberculosis was induced to L-form strain;②L-forms of Mycobacterium tuberculosis from clinical specimens were separated;③Via acid-fast staining, fluorescent staining, electron microscopy examination, the tuberculosis antigen detection and genetic testing methods induced and clinical isolates of Mycobacterium tuberculosis L-form were identified;④Prepare susceptibility medium containing first-line antituberculotics and Aliazrin medium;⑤Mycobacterium tuberculosis L-form was inoculated in the medium containing antituberculotics,then cultured;⑥DNA from the induction and clinical isolates of Mycobacterium bacillary L-form strain was extracted and related resistance gene was sequenced, its links with phenotypic variation were analyzed;⑦L-form of Mycobacterium tuberculosis is inoculated in Aliazrin containing medium and cultured, the effect of Aliazrin on L-form of Mycobacterium tuberculosis was observed.Results①L-form induction model of Mycobacterium tuberculosis was established successfully;②Phenotypic variation of Mycobacterium tuberculosis L-form could cause the resistance of the M. tuberculosis, while induced L-form maintained unchanged gene sequence does not change the case of Mycobacterium tuberculosis resistant to isoniazid, rifampicin, ethambutol and streptomycin;③Aliazrin in drug concentration640μ,g/ml, have a noticeable effect of inhibition against Mycobacterium tuberculosis L-form.Conclusion Phenotypic variation and drug resistance of L-form of Mycobacterium tuberculosis has certain connections; Aliazrin has significant inhibitory effect against Mycobacterium tuberculosis in vitro.