Primary Culture And Identification Of The Olfactory Ensheathing Cell In Muscle Basal Lamina Tissue Engineering Scaffolds From Adult Rats

1. Objective:To observed the multiplication of the olfactory ensheathing cell planting in muscle basal lamina(MBL)2. Method:Choose10both male and female littermate and24month of age SD rats to obtain the bilateral olfactory bulbs and olfactory mucosa at1/3nasal septum, Purify the olfactory ensheathing cells(OECs) from olfactory bulbs and olfactory mucosa according to differing rates of attachment of the various harvested cells types and arabinosylcytosin method. Forskolin and bFGF were added into the cultures. Hochest33342marked the purification of OECs transfer in muscle MBL praper with the chemical and physical approach. To observe the3、5、7、9days OECs in MBL biological scaffolds cultured in vitro for chemical identification.3. Conclusion:Blue sign Marked different source of OECs with hochest33342were implanted the MBL biological scaffold multi-cultured5day were distributed evenly in the MBL in immuno fluorescence microscope, the MBL of OECs were multi-cultured7days density higher than5days, cultivate to9days, observe the MBL OCEs density within the7days has no obvious change, and two different source of OECs no difference.4. Discussion:From adult rat olfactory bulb, cultivate the nasal mucous membrane separation and purification of olfactory ensheathing cells implanted in muscle membrane tube joint training, training to olfactory ensheathing cells in muscle membrane tube, on the7th of olfactory ensheathing cells density peak is in phase balance, are no longer as density increased with the extension of incubation time.