Effects And Mechanism Of MiR-378on Hepatocellular Carcinoma Cell Invasion And Migration Potential

Background and ObjectiveMicroRNAs are non-coding RNAs of about22nucleotides in length that function asnegative regulators of gene expression through inhibition of target mRNA translation ordegradation of target mRNA to participate in a wide variety of biological processesincluding cell development, metabolism, proliferation, differentiation and oncogenesis. Onthe basis of preliminary study which showed that serum miR-378was significantly lowerin related HBV of hepatocellular carcinoma (HCC) than that in healthy control, andup-regulated expression of miR-378significantly inhibited HCC cell proliferation andcolony formation by directly targeting of IGF1R. We designed a study to investigate theeffects of miR-378on HCC cell invasion and migration potential, and to explore itsmechanism.Method1、HepG2and hepG2.2.15cells were transfected with miR-378mimics or miR-378inhibitors by Lipofectamine. HepG2and hepG2.2.15cells transfected with negativecontrol(NC) or inhibitor negative control (inhibitor NC) were cultured as negativecontrols.Cell invasion potential were evaluated by transwell invasion assay. Cell migratingability was detected by transwell migration assay and wound-healing assay.2、Constructed a plasmid vector for the overexpression of IGF1R and co-transfectedhepG2and hepG2.2.15cells with miR-378mimics and IGF1R. Co-transfected withmiR-378mimics and control vector group (CON) were cultured as negative controls.Observe whether miR-378inhibits HCC cell migration and invasion by directly targetingof IGF1R. Results1、Transwell invasion and migration assay showed that compared with NC group,there was a significant reduction in cell invasion and migration capacity after hepG2andhepG2.2.15cells transfected with miR-378mimics for72h. For hepG2cells, the rates ofinvaded or migrated cells (20×10magnification) were0.486or0.459in miR-378mimicstransfectant compared with the NC transfectant, respectively(P<0.01). For hepG2.2.15cells, the rates of invaded or migrated cells were0.582or0.566in miR-378mimicstransfectant compared with the NC transfectant, respectively(P<0.01). While no significantdifference of cell invasion and migration were observed after cells were transfected withmiR-378inhibitors compared with the inhibitors NC transfectant for72h in both hepG2and hepG2.2.15cells (p>0.05).2、Wound-healing assay showed that significant reduceed cell migration was observedin miR-378mimics transfectant compared with the NC transfectant after the scratch inboth hepG2and hepG2.2.15cells (P<0.01).3、Significant enhanced cell migration and invasion were observed after hepG2andhepG2.2.15cells which co-transfected with miR-378and IGF1R when compared with themiR-378and CON co-transfectant (p<0.01).Conclusion1、MiR-378inhibits HCC cell invasion and migration.2、MiR-378inhibits HCC cell migration and invasion by directly targeting of IGF1R.