Database Construction And Functional Analysis For Prostate Cancer Associated Amino Acid Variations And SNPs

Prostate cancer (PCa) is a common malignancy that develops in male reproductivesystem, with its genetic mechanism still unclear as yet. In recent decades, a number ofmolecular and genetic events about PCa have been reported, including mutation, indel,duplication, amplification, methylation, and polymorphism. The variant data plays animportant role in revealing biological mechanism of PCa, while it is quite time-consumingto get them. At present there is no professional database focusing on collecting PCaassociated variations, which demands the construction of a database meeting therequirements. As a result, we searched for and read literatures from PubMed to retrievethese variation data and finally constructed a database named "PCaMDB"(http://www.ibio-cn.org/PCAmutbase/home.php/).This database is based on LOVD. Wecollected information about PCa samples and variations reported in literatures. InPCaMDB, users can search for and download variant data. Furthermore, users can submitnew variants to our database. We also constructed another database to collect prostatecancer associated SNPs.In order to analyze the relationship between the variations and PCa, we selected fiveproteins from PCaMDB to do some research about protein stabilization and conservation.We found that31.6%of the mutations occurred in stabilization center and40%of themutant residues were conserved, among which six mutated residues were highly conservedand buried. In all, we identified several key prostate cancer associated mutations.