The Impact Of Regulation Of The Smad Signaling Pathway By Smurf1in Tongue Cancer Cell

Tumor occurrence and development is a complex process with multi-step,multiple factors and multiple genes.It manifest as blocked of tumor cell apoptosis,disorders of differentiate,uncontrolled of proliferate. Below normal physiology circumstance,cell proliferation, differentiation, maturation and poptosis tightly regulated by a variety of positive and negative regulatory factors, positive and negative regulator of signal in dynamic equilibrium,maintain the stability of the physiological state. One of the mechanisms of tumorigenesis is maybe that the reaction of the cells to negative feedback inhibitor changed so that cells break away from the normal growth regulation. In recent years, many studies have shown that the abnormal of BMP-Smad signal transduction pathway is closely related to the occurrence and development of many tumors.BMP,Smads and the quality and quantity of the target gene changes are closely related to human tumors. Ubiquitin-proteasome degradation system widespread in a variety of eukaryotic cells, participate in a variety of physiological process of cells. As the core members of the regulation of degradation of system, more and more focus has been put on the functions of E3ubiquitin ligase. E3ubiquitin ligase Smurf1,as an important part of this system,participate in the process of signal transduction of BMP pathwan in the tissue.In this study,we using liposomal transfection technology to produce high expression of Smurfl protein in the tongue cancer cell, using the Western blot technique to study the content of Smadl, and Smad5protein in Tongue cancer cells,using the assay of MTT to test its influence upon tongue cancer cells.so that,maybe we can seek effective methods of gene therapy for the treatment of tongue cancer.Objective:Research the influence of Smad1and Smad5by overexpression of Smurfl. Research the influence of tongue cancer cell by overexpression of Smurfl.Methods:Transfect the plasmid which containing the Smurfl gene into the tongue cancer cell line Ca127by using liposomal transfection technique. Using the Western blot technique to study the content of Smadl, and Smad5protein in Tongue cancer cells, using the assay of MTT to test its influence upon tongue cancer cells which plasmid containing the Smurfl gene was transfected in.Results:1.The plasmid contained the Smurfl gene was transfected in the tongue cancer cells.The plsmid which contains Smurfl gene can express green fluorescent protein, After transfection,we observed the fluorescent protein in the fluorescence microscope,this shows that the plasmid has successfully transfected into tongur cancer cells.2.After transfection.the Smurfl protein expression quantity increased significantly campared with the control group, the Smad1protein expression quantity decreased significantly campared with the control group, the Smad5protein expression quantity decreased significantly campared with the control group.3.We used the MTT method to detect the tongue cancer cells after transfection,and we found that the proliferation activity of the tongue cancer cells was higher than the control group.Conclusionl.With Western Blot detected we found that the content of the Smurfl protein increased compared with control after transfection,the content of the Smadl and Smad5protein decreased,that shows that the high expression of Smurfl inhibit the expression of Smadl and Smad5.2.MTT test shows that the high expression of Smurfl protein enhance the proliferation activity of the tongue cancer cell. 3.The hige expression of Smurfl may be influence the proliferation activity of tongue by inhibit the content of Smad1and Smad5protein.