The Pharmacological Effects Determination Caulis Aqueous Extract Of Pharmacokinetic Parameters

The pharmacological effect method pharmacokinetic-pharmacodynamicAn approach. That the pharmacological effect intensity indicators for thedetermination of the dose-response relationship, and-effect relationshipconverted to time-dose relationship, without solving the pharmacokineticparameters determination of plasma concentration. Pharmacological effectmethod is described in the body save the amount of the drug by the process ofthe drug in vivo, most drugs, its pharmacological effect and bloodconcentration Movement parallel relationship between pharmacological effectmethod is closer to the in vivo the real situation.Source of acutum （Caulis Sinomenii） Menispermaceae plant ivy or woolivy cane. This product is mainly composed of Sinomenine, acutum alkali,double Sinomenine on the which Sinomenine its main active ingredient, whichhas pharmacological effects such as analgesia, sedation and local anesthesia.As a single herb, acutum its active ingredients is more clear, its preparationprocess, quality standards and efficacy studies.The subject application pharmacological effect method to study thepharmacokinetic parameters of the water extract acutum Sinomenine. Thinlayer chromatography qualitative identification of its High PerformanceLiquid Chromatography Determination of content; the applicationpharmacological effect method of Sinomenine pharmacokinetic parameters in vivo by the use of modern scientific techniques and methods, the water extractacutum Sinomenine when the evaluation of the process; relatively complexingredients of Chinese medicine, the application of pharmacological effectmethod to study there are some difficulties, in order to ensure the validity andaccuracy of the method of experimental methods of traditional Chinesemedicine pharmacological effects, the first choice of the analgesic effect ofstrong chemical drug diclofenac sodium as determination of thepharmacokinetic parameters of a model drug, the pharmacological effectmethod, parallel-controlled trial, theoretical and experimental basis for theapplication of traditional Chinese medicine pharmacological effect methodpharmacokinetic parameters.This article pharmacological effect method measured thepharmacokinetic parameters can reflect more realistically the Sinomeninedynamic in the body, the more realistic evaluation the intrinsic quality acutumand clinical applications.1Caulis aqueous extract of qualitative and quantitative researchAccording to TLC tests, with toluene-ethyl acetate-methanol-ammonia （2:4:2:1） as developing solvent exhibition from the12cm removedry spray to dilute bismuth potassium iodide solution resultssinomenine goodseparation. TLC for the test in the position corresponding to the referencesolution was the same color orange-red spots, negative samples withoutinterference.Experiments for high performance liquid chromatography to determinethe content of sinomenine. Silica gel as a filler to Octadecylsilanized;methanol-phosphate buffer （55︰45） as the mobile phase; detectionwavelength264nm, the number of theoretical plates the sinomenine peak ofnot less than4000. Methodological studies have shown that the above methodis simple, reliable, and can be used for the qualitative and quantitative determination of the appropriate ingredients in the formulation.2The diclofenac sodium pharmacological effect methodDiclofenac sodium as a model drug, as an indicator of analgesic effect,the use of the method for the determination of pharmacological effectsdiclofenac pharmacokinetic parameters in mice. Diclofenac results in theapparent dynamic processes of the stock after administration to comply withthe one-compartment open model, the main pharmacokinetic parametersmeasured: Ke=0.36h^-1ï¼›Ka=0.804h^-1ï¼›T1/2（Ke）=1.925hï¼›T1/2（Ka）=0.862hï¼›Tmax=3.601hï¼›Cmax=12.67mgï¹'kg^-1ï¼›V/F=1.06(mgï¹'kg^-1)ï¹'(mgï¹'kg^-1)^-1ï¼›CL/F=0.38(mgï¹'kg^-1)ï¹'[(mgï¹'kg^-1)·h]^-1ï¼›AUC=31.33(mgï¹'kg^-1)·h。3Study of the pharmacological effects of aqueous extract of CaulisCaulis water extract as a model drug, as an indicator of analgesic effect, topharmacological effects Determination sinomenine Caulis water extractkinetic parameters of the drug in mice. One-compartment open model resultsCaulis aqueous extract of the apparent dynamic processes in the stock afteradministration, the main pharmacokinetic parameters: Ke=0.227h^-1ï¼›Ka=2.998h^-1ï¼›T_{1/2（Ke）}=3.05hï¼›T_{1/2（Ka）}=0.231hï¼›T_max=3.950hï¼›C_max=75.40mgï¹'kg^-1ï¼›V/F=0.53(mgï¹'kg^-1)ï¹'(mgï¹'kg^-1)^-1ï¼›CL/F=0.12(mgï¹'kg^-1)ï¹'[(mgï¹'kg^-1)·h]^-1ï¼›AUC=1454.74(mgï¹'kg^-1)·h。...