Detection Of TERC Gene Amplification In Differential Diagnosis Between CIN1and CIN2Tissue

Objective: Due to the spread of HPV detection, as well as people tocombat cancer awareness, the incidence of cervical intraepithelial neoplasia(CIN) showed an increasing trend in recent years.CIN as a group of cervicalprecancerous lesions, their progress to cervical cancer is a chronic process,maybe it would be cured completly that as earlier as impossible diagnosis andappropriate treatment is entirely possible to prevent the development of cervicalcancer.Gynecological clinical principles treatment of CIN：CIN1can be takento conservative treatment or follow-up observation，however the CIN2needLEEP knife ring conization surgical treatment, biopsy is the correct diagnosisof gynecological clinical CIN1and CIN2level, guidance the meaning isobvious.Pathological diagnosis, however, is the method of morphological andexperience, that sometimes due to the influence of human factors, and thelesion not typical of itself.It is difficult to CIN1with CIN2accurately identifyout, which in some cases lead to inadequate treatment orovertreatment.Therefore urgently need for a sensitivily, accuratly and stabllymethod or indicators to assist pathological diagnosis.In recent years researchfound that cervical squamous epithelium from precancerous lesions to cervicalcancer,almost all of the long arm of chromosome3copy number increaseTERC gene copy number.Another study also confirmed that cytologicaldetection of TERC gene sensitivity and specificity in the identification ofhigh-grade cervical precancerous lesions and low-grade precancerous lesionsare more than90%.By inference detection of TERC gene copy numberincrease help CIN1or CIN2level’s accurate diagnosis.In our previous tissuemicroarray study of cervical cancer and CIN found the similarity conclusions.On the basis of the homemade cervical tissue，in this study TERC genewere detected by FISH to investigate the increase in copy number of the CIN1 and CIN2organization in the circumstances。And in accordance with the TERCgene test results were more clearer role in the diagnosis and differentialdiagnosis of CIN1/CIN2.Its practical value in the clinical pathology on thedetection of TERC gene are discussed.Methods:1A total of153cases cervical excised tissue were collected from thePeople’s Hospital of Hebei Province, what including20cases of normalcervical squamous epithelial tissue samples,55cases of CIN1and55cases ofCIN2, CIN1/CIN2of tissue samples from23patients.2Put153cases cervical tissues by manual tissue chip technique into arecipient paraffin block,and then fusing, slicing, gaining and baking,and madeinto tissue chip.3Using fluorescence in situ hybridization detection of TERC genewhether amplification or not in normal cervical squamous epithelium and CINorganization.4SPSS16.0statistical software for statistical analysis, the α=0.05levelof inspection, a statistically significant when P <0.05.Results:1The tissue microarray Preparation and staining resultsTotal production based on the topics design requirements and test methodsfor three8×9,6×7-intensive array of tissue microarray neatly tissue latticedisplacement phenomenon, including seven organizations lack of sites, ninetissue sites without meaningful, other sites are meaningful, and the final resultswere obtained meaningful sample of137cases (20cases of normal cervicalsquamous epithelial tissue,46cases of CIN1-level,49cases of CIN2-level,22cases of CIN1/CIN2-level), the morphology of the tissue site can be observedrate of89.54%. HE staining no off-chip, ectopic and wrinkles, fluorescence insitu hybridization signals are clearand a clean background.2TERC gene in cervical epithelial lesions amplificationDetecting the amplification of TERC gene use fluorescence in situhybridization (FISH) including20cases of normal cervical squamous epithelial tissue and46cases of CIN1level and49cases of CIN2-level, and22cases ofsquamous epithelial tissue of CIN1/CIN2.The results showed that: the TERCgene in normal cervical squamous epithelium group no amplification of theTERC gene and the CIN1level in TERC gene amplification is15.2%(7/46),47%(23/49). There is a large number of triploidand，tetraploid and aneuploidamplification. The differences between two groups were statistically significant(p <0.05). The CIN1/CIN2grade lesions TERC gene amplification was27.8%(5/22).Between any two groups: normal cervical squamous group and CIN1level group difference was not statistically significant (p>0.05); groups ofnormal cervical squamous epithelial groups with CIN2level of groupdifference was statistically significant (p<0.05); the CIN1level group andCIN2group difference was statistically significant (p<0.05).TERC geneamplification in the identification of CIN1lesions with CIN2lesions weresensitivity46.9%and specificity84.7%and accuracy of65.0%and a positivepredictive value of76.6%and negative predictive value of60%.Conclusions:1FISH detection of TERC gene amplification in normal cervicalsquamous epithelial tissue without amplificationand a small amount ofamplification in CIN1-grade lesions CIN2lesions were amplified significantlyincreasedand and triploidand tetraploid and polyploid. CIN1grade lesions andCIN2level of lesions in the differential diagnosis of significant clinical value.2FISH detection of the TERC gene amplification higher specificity,positive predictive value, and accuracy in the identification of CIN1gradelesions and CIN2retinopathy and clinically predictive value for determiningthe progress of CIN lesions.3Amplification TERC gene detected by FISH and difficult to identifythe morphological CIN1/CIN2grade lesions increase TERC genetic testing, theresults will make more clearer, providing accurate and reliable basis forgynecological clinical treatment.4Mannual tissue chip technique is simple, cost-effective, and reliable.This technique can provide a highly efficient, high-throughput mechanism for someresearch.