| Objective: With the development of the industry, the emissions ofindustrial pollutants, the incidences of cancers, metabolic diseases and maleinfertility are increasing year by year. In numerous experimental studies, itwas found that the increasing of incidence of these diseases and environmentalpollutants emissions is closely related. Therefore, environmental pollutionshave been one of the global problems to be solved. In this context,environmental endocrine disrupters(EEDs) have become major concerns ofdomestic and foreign scholars. Bisphenol A (BPA) is an environmentalendocrine disrupter, widely used in the industry. The concern of this chemicalwas increasing, due to its weak estrogenic and strong anti-androgenic effects,and the strong reproductive and developmental toxicity. Moreover, the declinein rate of male fertility leads scientists to focus more on the environmentalendocrine disrupters, bisphenol A.We exposed the SD rats to bisphenol A(BPA) to observe the chemical’seffects on the rat testis caused by accumulation of toxic. The expressions ofBcl-2, CytC and Caspase-3which are important in germ cell apoptosis, weredetermined, and the deformation of sperm was observed.In short, we investigated the effects of BPA on male spermatogenic cellapoptosis, established evidences for clinical prevention and treatment of maleinfertility.Method：1Animals: Forty male SD rats was offered by animal center of HebeiMedical University (8weeks of age; body weight175-210g). All rats werekept in individual cages with free access to normal rat diet and tap water for1week of acclimatization.2Grouping and Modeling:40SD rats were randomly divided into four groups (control group, low-dose group, middle dose group and high dosegroup) and given BPA solved in corn oil0、2、20、200mg/Kg/day for8weeks.Weighed weekly, and timely adjust the dose of BPA according to the weightof each group of SD rats.3Specimen processing: After exposure, animals were weighed and killedby decapitation. Testis and epididymis were removed quickly and washedthoroughly with normal saline to remove the contaminating blood and thenimmediately processed for morphological studies. Data are expressed asmeans±SD. Statistical analyses were performed using SPSS13.0.Result:1Day-to-day activities observed in SD rats: With bisphenol A gavageongoing, symptoms of irritability was observed in high-dose group SD rats.Messy hair, reduced activity, less eat, loose stools, etc. were also observed.Compared to the control group, there are no significant differences wereobserved in low and middle dose groups.2The effect of BPA on SD rats body weight and testicular weight:Compared with the control group, slow weight gain, and low testis coefficientwere observed in the midlle/high-dose group. But no significant differenceswere observed between low-dose group and control group.3The expression of Bcl-2、CytC and Caspase-3in Different dose groupsof SD rats exposed to BPA: Bcl-2expression was observed in cytoplasm ofspermatogonial cells, with the exposure dose increasing, the expression ofBcl-2decreased. Compared with the control group, the expression of CytCand Caspase-3in midlle/high-dose group were up regulated.4Observing SD rat epididymis sperm in different groups with microscopy:No significant differences were observed between the control group andlow-dose group in sperm count, sperm motility, and sperm malformation rate.Between high-dose group and the control group, notable difference wereobserved concerning sperm p head deformity and tail deformity.Conclusions: Following prolonged exposure to BPA, weight loss,metabolic disorders, testicular dysfunction, decreased sperm density, decreased vigor, and shape deformity were observed. The expression of Bcl-2was down regulated, and CytC and Caspase-3were up regulated. Wespeculate that the BPA induces spermatogenic cell apoptosis. |
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