| Objective Inducing the diabetic rat model by streptozotocin (STZ) and intervening them by using of triptolide (TPL), at the same time, testing the expression level of RANTES in rat kidney, to investigate the effects of triptolide on the renal tissue of diabetic rats and its possible mechanisms.Methods After1week adaptive feeding, SPF SD rats were randomly divided into Normal contro group(NG) and Model group according to the random number table. Continued feeding for4weeks, the rats from Model group were induced diabetic model by one-time intraperitoneal injection of streptozotocin, after the success of modeling, the modeled rats were randomly divided into Diabetic model group(DM), Low dose triptolide treatment group(T1), High dose triptolide treatment group(T2) according to the random number table once more. The T1group were given0.2mg·Kg-1, T2group0.4mg-Kg-1triptolide by gavage. Five rats of each group were randomly selected and the organ specimens were removed in the fourth, eighth and twelfth week of the experiment. Five rats of each group were randomly selected out、 killed and taken out of the organ specimens. Body weight, kidney weight,24hours urinry albumin(24hUAL), plasma glucose, total cholesterin, triglyeride, the function of liver and kidney, WBC and HbAlc were determined. The mRNA expression of RANTES in kidney tissue was assessed by reverse transcription-polymerase chain reaction(RT-PCR).The protein expression of RANTES in kidney tissue was determined by Enzyme linked immunosorbent assay(ELISA), and renal tissue was observed by routine pathological examination.Results Compared with the NG group, body and kidney weight in the DM group were significantly attenuated in the fourth, eighth, twelfth week. But24hUAL was evidently raised in the DM group. The expresson of RANTES in the DM group was also markedly increased than that in the NG. group. Glomerular sclerosis index (GSI) and renal interstitial fibrosis index (RIFI) were evidently inhibited in triptolide treatment group, which were pronouncedly elevated in the DM group. Compared with those in the fourth week, the expression of RANTES, GSI and RIFI in the twelfth week were significantly increased in the DM group, which were evidently decreased in the Triptolide treatment group.Conclusion Triptolide could not only inhabit the transient expression of RANTES but also improve the glomerular sclerosis and renal interstitial fibrosis in the renal tissue of diabetic rats, In addition, Triptolide could significantly decrease the level of24hUAL in rats. |
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