Fas Protein And Ki67Protein Expression And Clinical Significance In Thymoma

Objective:1Fas expression in thymus tumour as a starting point, to learn moreabout its effect in the process of Fas in thymoma and mutual relationship; Theexpression of Fas in thymic tumor invasion and metastasis process functionand mutual relationship; And the expression of Fas in thymoma is involved inthe pathogenesis of myasthenia gravis, further for thymoma in clinical work,and the treatment of myasthenia gravis looking for new ways.2Ki67expression in thymus tumour as a starting point, furtherknowledge about the role of Ki67in thymoma, Ki67expression andcorrelation of thymic tumor pathological grade, Ki67role in thymic tumorinvasion and metastasis process, Ki67expression in thymus tumour is relatedto whether the occurrence of myasthenia gravis, to provide basis for thediagnosis and prognosis estimation of thymoma.Methods:Select the thymus gland neoplasm resection after pathology diagnosis ofthymoma (80cases), including thymic carcinoma (14cases had A16cases,AB, type B1in16cases,8cases type B2B3type6cases, type C,20cases),because the normal thymus excision of congenital heart disease surgerycutting exposure to10cases as control group. Fas was evaluated byimmunohistochemical staining SP method and expression level of Ki67,chi-square test and Spearman rank correlation analysis of statistical methodfor determining both with thymic tumor pathological type, clinical stage, therelationship of myasthenia gravis.Results:1Fas expression in thymus tumour positive rate is:56.3%(45/80), in thenormal breast tissue was positive rate was90%(9/10), the difference was statistically significant (Fisher’s exact probability: bilateral=0.046, P <0.05),indicating the occurrence of thymoma and Fas protein abnormal expression inthymus.2Fas in benign (type A, AB), malignant Ⅰtype (B1, B2, B3), malignanttype2(C thymic carcinoma, namely) positive expression rate of thymomawere73.3%(22/30),60%(18/30),25%(5/20). During the difference hasstatistical significance (χ squared=11.666, P=0.03, P <0.05), and throughthe Spearman correlation analysis (r=0.361, P=0.361, P <0.05), negativecorrelation between them, and prompt positive expression rate of Fas as cellsmalignant degree increased gradually reduced.3Fas in clinical Masaoka staging Ⅰ, Ⅱ, Ⅲ, phase Ⅳpositive expressionrate of80%(16/20),65%(13/20),38.1%(8/21),42.1%(8/19), which wasstatistically significant difference (χ squared=9.564, P=0.023, P <0.05),and through the Spearman correlation (r=0.318, P=0.318, P <0.05),suggesting the expression of Fas as thymoma late clinical stage and graduallydecreased.4Fas in thymoma myasthenia gravis with thymoma associated withmyasthenia gravis group (Osserman stages: stage Ⅱ Ⅰ+a+Ⅱb)expression positive rate was88.2%(15/17),58.1%(25/43), which wasstatistically significant difference (χ squared=4.966, P=0.026, P <0.05).Prompt thymoma in the occurrence of myasthenia gravis associated with theabnormal expression of Fas.5Ki67in thymoma expression positive rate was55%(44/80), allexpressed in normal breast tissue in the negative (0/10), the difference wasstatistically significant (Fisher’s exact probability method, P=0.000, P <0.05). Explain the occurrence of thymoma associated with the expression ofKi67.6Ki67in benign (type A, AB), malignant Ⅰ type (B1, B2, B3),malignant type2(type C, namely thymic carcinoma) positive expression rateof thymoma were33.3%(10/30),73.6%(22/30),60%(12/20), withstatistically significant difference (χ squared=9.966, P=0.007, P <0.05), and through the Spearman correlation (r=0.261, P=0.019, P <0.05), bothare positively related, prompt Ki67as cells malignant degree is higher andhigher.7Ki67in thymic tumor clinical Masaoka staging Ⅰ, Ⅱ, Ⅲ, Ⅳperiodin the positive expression rate is respectively:20%(4/20),30%(6/20),76.7%(16/21),94.7%(18/19), which was statistically significant difference (χsquared=30.881, P=0.000, P <0.05), and through the Spearmancorrelation analysis (r=0.578, P=0.000, P <0.05), suggesting Ki67inthymoma expression was positively correlated with clinical stage, the laterclinical stage with thymoma is increased gradually.8Ki67in group with myasthenia gravis myasthenia gravis thymoma inthe positive expression rate was47.1%(8/17),55.8%(24/43). Meanwhilethere was no statistically significant difference (χ squared=1.409, P=0.235,P>0.05). Prompt thymoma in the expression of Ki67with myasthenia gravishas important significance, no matter whether the merger of myastheniagravis, thymic tumor cell proliferation activity of the difference is notobvious.9Fas and Ki67association study found in thymic tumor, the expressionof Fas and negatively correlated with the expression of Ki67(r=0.443, P=0.443, P <0.05).Conclusion:1Fas in thymoma and there are differences between the positiveexpression rate of normal thymus, hints of thymoma and normal due toabnormal expression of Fas apoptosis is related to the disorder.2Fas expression with the increasing of thymic tumor malignant degreeof pathological classification obviously decrease trend, shows that theexpression of Fas gradually reduced to gradually increase the thymuspathology grade tumor escape from immune surveillance ability increasesgradually, and with the reduction in cell apoptosis, prompt Fas lowerexpression of thymic tumor malignant degree is higher.3The expression of Fas reduced gradually along with the later clinical stage Masaoka stage, low tip Fas thymic tumor cells are more likely toescape immune surveillance of infiltrating embellish and transfer.4Fas expression in myasthenia gravis myasthenia gravis with thymomain the two groups have significant differences, in tip Fas is an importantfactor of myasthenia gravis, consistent with the reports.5Ki67were not expressed in normal breast tissue, the thymus glandtumors with high expression, prompt the thymoma is the result of abnormalthymus cells proliferation.6The expression of Ki67with thymoma higher malignant degree ofpathological grading, hint is its judgment thymic tumor malignant degree ofsensitivity index.7In thymic tumor Masaoka staging Ki67protein expression with theclinical stage and increased later, prompt high Ki67express thymoma is moreprone to invasion and metastasis.8Ki67no important role in the pathogenesis of myasthenia gravis,explain the occurrence of myasthenia gravis or not, does not affect theactivity of thymus tumor cell proliferation.9The expression of Fas in thymoma and negatively correlated with theexpression of Ki67, shows that thymus tumor occurrence, developmentprocess, reduce tumor cell apoptosis accompanied by excessive proliferationof the cells. As indicators of cell proliferation Ki67antigen and apoptosisindex of Fas antigen combination, can well reflect the thymoma imbalancebetween cell proliferation and apoptosis.