| Objective: To study the acute toxicity of Shaogenfang and determine thescope of security, in order to provide the experimental basis for the safety ofclinical medicine. To compare pharmacodynamic effects of Shaogenfang of itsgranule and water decoction, in order to provide a reference for theShaogenfang formulations conversion. To research the Pharmacologicaleffects of different drug pairs of Shaogenfang and its Chinese medicine theory.Methods:1Different doses of Shaogenfang were orally given to mice,observingtoxic reaction and recording the number of death of mice,determining themaximum administration doses in mice.2The method that mice ear swelling induced by xylene and hot plateanalgesia in mice were used in this study,to observe and compare theanti-inflammatory swelling and analgesic effects of different dosage forms ofShaogenfang.The mice ear swelling induced by xylene:80male mice were randomlydivided into eight groups by weight (N=10).Group A: The high concentrationof granules group. Group B: Granules moderate concentration group. Group C:The low concentration of granules group. Group D: Water decoction of highconcentration group. Group E: Water decoction of moderate concentrationgroup. Group F: Water decoction of low concentration group. Group G:Positive control group (given Yunnanbaiyao). Group H: Blank control group(given distilled water).Each group were administered once daily time, volume0.2ml/10g, consecutive7days.30min after last administration, mice weresacrificed, chisel wafer in the left and right ears of the same parts used9mmMyotis puncher, then weighed. And calculate Swelling rate. The method of hotplate analgesia: First screened80qualifying mice through the pre-experiment, started the formal experiment after24h.The mice were placed on the hot plate(55℃),to determine basic pain threshold of each mouseby observing the timeof the pain response, then randomized and administered, the specific contentin accordance with the previously described.1h after the last administration,began to observe. Recorded the mice pain threshold in30,60,90,120min. Toprevent foot scald, calculated in accordance with60s if the pain threshold than60seconds.3The method that mice ear swelling induced by xylene was used in thefirst study. By comparing the rate of ear swelling of the mice, and to show theAntiinflammation-Swelling effects of different drug pairs of Shaogenfang andthe difference among the groups. The method that hot plate analgesia in micewas used in the second study.By comparing the increase of pain threshold, toshow the analgesic effects of different drug pair of Shaogenfang and thedifference among the groups(The two experimental methods are the same asdescribed in method2). The method that mice writhing also was used in thesecond study.By observing the number of writhing in different groups, toshow the analgesic effects of different drug pair of Shaogenfang and thedifference among the groups, and calculated the pain inhibition rate of allgroups. By using chemical colorimetric and TBA colorimetric, to detect NOand MDA of mice serum, and then discussed the analgesic mechanism.The mice writhing:80mice were randomly divided into eight groups bysex and weight (N=10).They were Shaogenfang group, RUSHAN highmoderate low concentration groups and RUSAN high moderate lowconcentration groups and blank control group (given distilled water). Eachgroup were administered once daily time, volume0.2ml/10g, consecutive7days.1h after last administration, injected0.6%glacial acetic acidintraperitoneal, volume0.1ml/10g.To observe the number of writhing indifferent groups within20min(stretching hindlimb, abdomen concave orraising the hips),then calculated the pain inhibition rate of all groups(thewrithing number of blank control group-the writhing number of each group/the writhing number of blank control group). 4By analyzing the pathogenesis of traditional Chinese medicine of acuteradiation esophagitis, to explain the reason of drug together.Results:1The obvious toxic reaction were not observed in experimental group,thechanges of mice weight were no significant difference compared with thecontrol group(P>0.05),the maximum administration doses was460g（/kg·d）,itwas equivalent to180times the clinical recommended dose of adult.2Both the granule and water decoction of Shaogenfang have the effecton inhibiting mouse auricle swelling. Different dosage forms all could makethe incubation period of pain induced by hot stimulation in mice wereprolonged(P<0.05).But between of the two dosage forms were no differencein pharmacodynamic effects(P>0.05); The effect of high concentration was thebest one in the different concentrations of two dosage forms(P<0.05);In theantiinflammation-Swelling,there was no difference in positive control groupand high concentration of Shaogenfang (P>0.05).In pain,the effect of highconcentration was better than the positive control group(P<0.05).3The medium concentration and the higher concentration of the two drugpairs of Shaogenfang had the effect on inhibiting mouse auricle swelling(P<0.05). Yu Luo group was better than Ru Gong group on the effect(P<0.05).The Shaogenfang had the best effect on inhibiting mouse auricleswelling. The medium concentration and the higher concentration of the twodrug pairs of Shaogenfang had the effect on the increasing of pain threshold(P<0.05).Ruxiang Shandougen group was better than Ruxiang Sanqi group onthe effect (P<0.05). The Shaogenfang had the best effect on increasing of painthreshold. Shaogenfang group,RUSHAN moderate high concentration groupsand RUSAN high concentration group are all can reduce the number of micewrithing(P<0.05), shaogenfang group and RUSHAN high concentration groupwere more obvious among them(P<0.01),and shaogenfang group was mostobvious(P<0.05). On the same concentration, RUSHAN moderate and highconcentration groups were better than RUSAN(P<0.05).The NO and MDA inserum were all decreased significantly in Shaogenfang and RUSHAN high concentration group (P<0.05),compared between them, shaogenfang was moresignificantly (P<0.05). The rest groups were also decreased in value, but nostatistically significant (P>0.05). On the same concentration, RUSHAN highconcentration group was more significantly than RUSAN high concentrationgroup on the reduction of NO and MDA (P<0.05).4From the pathogenesis and treatments of traditional Chinese medicineon acute radiation esophagitis and modern pharmacological effects, the studyselected drug pairs are reasonable.Conclusion:1Shaogenfang is safe in clinical doses.2Two dosage forms of Shaogenfang had the similar pharmacodynamic effects in above-mentioned pharmacodynamic markers; Compared with the positive control group (YunNanBaiYao group),the effects of highconcentration of Shaogenfang was obvious.3Both the two drug pairs of Shaogenfang had the different degrees effecton inhibiting mouse auricle swelling, but compared with Shaogenfang, theeffect was lower, and YULUO group was better than RUGONG. On thedose-effect relationship, the group of higher concentration was better than thegroup of medium and lower concentration on the effect. Both the two drugpairs of Shaogenfang had the different degrees effect on increasing of painthreshold, but compared with Shaogenfang,the effect was lower, andRUSHAN group was better than RUSAN. On the dose-effect relationship, thegroup of higher concentration was better than the group of medium and lowerconcentration on the effect. The analgesic mechanism of Shaogenfang andselected drug pairs, may be the decrease of NO and MDA.4The study selected drug pairs of Shaogenfang are reasonable, accordthe theory of traditional Chinese medicine. |
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