| Background and ObjectiveCervical carcinoma is the most common malignant tumors in female bodies, and the incidence rate of which is slightly higher than breast cancer. Every year83%of new cases are in developing countries, with the tendency that the age is getting younger. Of all the cases, cervical squamous cell carcinoma is the most common type, this situation being severe, thus threatening the health and life of the female in our country. The continuous infection of High-risk Human papilla virus (HPV) makes up the major cause of cervical carcinoma; however, it is not the only reason. The incidence and development of it is the result of various reasons, with several phases and steps, on this account, indicators concerning clinical significance should be consulted. Recently researches have found that metastasis-associated gene1(MTA1) is a sort of gene related to tumor metastasis; also, researches on the SLP-2(stomatin-like protein2) protein to various tumor have found that to various tumors of proper pairing, the expression of SLP-2in tumor tissue is Overexpressed, while there exists not much research of it in cervical carcinoma tissue. As a result, this paper focuses on the following issues:whether the expression in tumor tissue is upexpressed or downexpressed; the relationship between SLP-2and clinic-pathological parameters, and whether SLP-2is relative to MTA1.Materials and methods: 1.Specimen origin:All samples are collected from the surgical excision tissue from May of2011to May of2012in the Second Affiliated Hospital of Zhengzhou University. The samples are divided as following:48cases of normal cervical tissue of total hysterectomy of uterine leiomyoma type(Group A);43cases of cervical intraepithelial neoplasia (CIN)(Group B);42cases of confirming pathological diagnosis of squamous carcinoma of the cervix without radiotherapy and chemotherapy before surgery(Group C). Samples of corresponding archival paraffin blocks in pathology department are also collected.2.Experimental methods:immunohistochemistry techniques is adapted to detect the expression and distribution of MTA1and SLP-2in different cervical tissues. The method of RT-PCR to detect the changes in MTAlmRNA and SLP-2mRNA levels.3.Statistical methods:SPSS17.0software package is adapted to analysis the data: both t test and one factor analysis of variance are adapted to examine the comparison of quantitative data,χ2to the comparison of rate, and2×2Correlation Analysis of paired data to relevance analysis. Inspection level:α=0.05(bilateral).Results:1.the expression of MTA1:in normal cervical tissues MTA1protein is mainly located in cell nucleuses while for CIN and MTA1in cervical carcinoma various protein location are found in the cell membranes, nucleuses and cytoplasm. The positive expression in Group A,B and C is overexpressed, and to same goes to the expression of MTAlmRNA. However the expression of latter in Group A is remarkably lower than Group B (P<0.05) and Group C (P<0.05). In Group B, the expression of MTA1is lower than Group C, but it has no statistical signification (P>0.05). the expression of MTA1in squamous carcinoma of the cervix has no relation to the age of the patient, clinical stages, degree of tissue differentiation, and the size of tumor (P>0.05), while it is highly related to lymphatic metastasis and the depth of myometrial invasion (P<0.05). the expression of MTA1will be upexpressed with the lymphatic metastasis of the patient and the deepening of the depth of myometrial invasion.2.The expression of SLP-2:in normal cervical tissues SLP-2is mainly located in cell membranes and cytoplasm, while for CIN and SLP-2protein in cervical carcinoma is mainly expressed in the form of cytoplasm staining. The positive expression of SLP-2protein is downexpressed in Group A, B and C, and the same goes to the expression of SLP-2mRNA. The expression of the latter in Group C is obviously lower than that of Group A(P<0.05) and B (P<0.05). In Group B, the expression of SLP-2is obviously lower than Group A(P<0.05). The expression of SLP-2bears no relations with the age of the patient, clinical stages, the existence of lymphatic metastasis, the depth of myometrial invasion, and the size of tumor (P>0.05), while it is relative to the degree of tissue differentiation. With the increase of the degree of differentiation, the espression level of SLP-2is also increase (P=0.033).3.The relativity of expression of MTA1and SLP-2in cervical carcinoma tissues: the expression of expression of MTA1and SLP-2in cervical carcinoma tissues is of inverse correlation(r=-0.393，P=0.037).Conclusion:1.The MTA1in cervical squamous cell carcinoma is of high expression, which is highly related to the lymphatic metastasis of tumors and the depth of myometrial invasion. The fact that MTA1is related to the occurrence and development of cervical carcinoma could be used as the index of the degree of malignancy of cervical squamous cell carcinoma.2.The SLP-2in cervical squamous cell carcinoma is of low expression, which is probably a new cancer suppressor gene. It is relative to the degree of tissue differentiation, however, further research is required to confirm the molecular mechanism of SLP-2in the occurrence and development of cervical squamous cell carcinoma.3.The expression of MTA1and SLP-2in cervical squamous cell carcinoma is of negative correlation, the high expression of MTAl and low expression of SLP-2is probably highly related to the occurrence and development of cervical squamous cell carcinoma as well as the biological behaviour. |
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