| BackgroundNowadays there is an obvious increase of accidental injuries in the society; We could see the number increase in scalds, chemical burns, electrical injury as well as other diseases. As for the treatment of the diseases mentioned above, there are amounts of treatments, of which Western medicine, traditional Chinese medicine, for external use dressings and folk remedies are the main approaches. However, there may be some deficiencies in the treatment process.For instance:there is a wide use of various antibiotics in clinical western treatments, which could easily cause drug resistance of bacterium and double infection when used for a long term. Besides, most antibiotics could bring with adverse drug reaction easily and have difficulty in killing super bacterium. In addition, State management of the use of antibiotics has become more and more strict. Therefore, there should be a sharp decrease in the application of antibiotics during the process of treatment.In the clinic treatment of traditional Chinese therapy, the curative effect is unconspicuous and the course of treatment is comparatively long.At present, the use of topical treatment of dressing, such as silver ions dressing and Medical fault pig skin has a better effect but the expense is extremely high. Some patients give up the treatment because of the high hospitalizaed costs.The folk prescriptions that handed down for ancestors hardly have any effect and lack effective scientific proof, which may delay the disease and in turn cause serious side effects.As for the shortcomings mentioned above in recent years, during the process of burning treatments, more importance has been attached on the exploitation and application of Chinese medicine paste. Chinese medicine paste is based on solid science, besides; it has the advantages of convenient preparation, significant effect, low cost and less side effects.ObjectiveCompound Chinese medicine ointment are compound preparation made up of many kinds of Chinese medicine. In order to study their effect on the process of burn wound healing, this study choose turok swine deep Ⅱ burn model in an objective test design. Observe swine deep Ⅱ degree burn wound, Under the action of drugs of the wound, measure MDA content and SOD activity in the tissue. In the meanwhile, The IL-6, endotoxin and the content of TGF were measured in serum. Observed were also the wound healing speed and healing quality. In order to discuss the effect of Compound Chinese medicine ointment on burn wound healing. Compared was its effect with Sulfadiazine silver at the same time.Summarized was the changing rule of the drugs on burn wound healing, the relationship between the burn wound healing and factors involved. Provided was necessary theoretical basis of the laboratory for the treatment of skin disease deep Ⅱ degree burn wound.In the process of experiment, we will explored a simple method of swine skin deep Ⅱ degree burn model.MethodsPrepare deep Ⅱ degree burn model with the symmetrical parts on the bake of12swines. The burn wound is4cm in diameter. Divided them were into four groups at randomly. Group A:daub Compound Chinese medicine ointment on the wound; group B:daub Sulfadiazine silver Ointment on the wound; group C:daub Jing wanhong on the wound. Group D:use normal saline to clean the wound every day, no any medical paste, with three swine’s in each group. Observe wound changes of the burn wound continuously. Used rotary drill was take2pieces of the wound tissues with the same size from every wound on each swine on the1st day, the7st day, the14th-day, and the21st day, the sample should have a distance of0.5cm from the wound edge.Extract2ml blood from ear vein and inject it into the sterile EP tube.Smash and centrifuge one piece. The content of MDA in the tissue and the vitality of SOD were measured, The blood and measure IL-6,TGF-βand endotoxin were centrifuged, the mechanism of burn wound healing was discussed, its speed and its quality.Fasten another piece in10%formaldehyde solution, then dehydrate and embed it into wax pieces. After the petrolin embed the tissue section, observe the changes of pathological histology in the process of burn wound healing in each group via HE stain.Results1. The content of MDA in each group, in the pig skin tissue of burn wound, is not obvious on the first day (P>0.05). After the use of medicine, the content of MDA in each tissue block is increasingly decreasing in comparison with that in the model set. The content of MDA in the group A is comparatively higher than group B on the second day. There exist great different between the two group (P<0.05).The content of MDA in the group A is lower than that of group C. There is great different between the two group (P<0.05), obvious difference exist as well compared with group D (P<0.05).MDA content in every tissue block reduced a bit and the difference are significant among groups.2. The vitality of SOD in wound pig skin tissue increases in every group on the first day, there is no obvious difference among groups (P>0.05).After treatment, the SOD vitality of tissue block in the treatment group is increasingly higher in comparison with that of model set. On the second day, SOD vitality in the group A is lower than group B. There are great differences between the two groups (P<0.05). SOD vitality in the group A is higher than group C. There are great differences between the two groups (P<0.05).There also exist great differences compared with group D (P<0.05).On the seventh day, the SOD vitality of tissue block continuingly increase, there are great differences in each group.3. The first day after burn, the content of IL-6in wound pig serum is apparently high er in each group than before but with no obvious difference compared with other groups. After treatment, the content of IL-6in experimental group is lower.On the second day, the content of IL-6in the group A is higher than that of group B, there are great differences between them (P<0.05). While the content of IL-6in the group A is lower than that of group C, there are great differences between them (P<0.05).There are also great difference when it is compared with group D (P<0.05).On the seventh day, the content of IL-6in wound pig serum decreases in each group, There are also great differences among groups.4. The content of TGF-βin wound pig serum is apparently lower in each group than before, there are no significant differences (P>0.05). After treatment, the content of TGF-p in drug groups is increasingly higher that of model set group. On the second day, the content of TGF-βin group A is lower than group B, there are significant differences (P<0.05). the content of TGF-P in group A is higher than group C, there are significant differences between the two groups (P<0.05), There are also great difference when it is compared with group D (P<0.05). On the seventh day, the content of TGF-βin each group continues to decrease; there are also great differences among groups (P<0.05)5.The content of endotoxin in wound pig serum is apparently higher in each group than before, there are no significant differences (P>0.05). After treatment, the content of endotoxin in drug group is increasingly lower in comparison with group D. On the second day, the content of endotoxin in group A is higher than group B, there are significant differences (P<0.05). the content of endotoxin in group A is lower than group C, there are significant differences between the two groups (P<0.05), There are also great difference when it is compared with group D (P<0.05). On the seventh day, the content of endotoxin in each group continues to decrease; there are also great differences among groups (P<0.05) Conclusion1.The stable swine’s scald wound with deep Ⅱ degree model can be produced by the heat gun in9seconds.2. Compound Chinese medicine ointment can promote the healing process of swine deep two degree burn and protect scald model properly.3. Compound Chinese medicine ointment can raise the vitality of SOD in wound tissue and decrease the content of MAD. It also has an significant effect in antioxidant.4. Compound Chinese medicine ointment can decrease the content of endotoxin in serum and control the release of IL-6inflammatory mediators level.5. Compound Chinese medicine ointment can stimulate βtransforming growth factor increase in serum... |
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