Effect Of Arsenic Trioxide On The Adult Neurogenesis In Mouse

Adult neural stem cells in clinical applications has broad prospects, its research has been a hotspot of neurological research in the world, a large number of morphological and behavioral experiments show that adult stem cells and learning and memory are closely related. In recent years, the arsenic poisoning damage to the central nervous system has aroused great attention, but the research of chronic arsenic poisoning of adult neurogenesis in adult mouse model has not been reported. Our experiment established chronic arsenic poisoning mice model and focuses on the cellular level study of the hippocampal formation in the subzone of dentate gyrus (SGZ), including the proliferation of adult stem cells speed and newborn cell viability, to clarify the relationship of the adult neurogenesis and chronic arsenic poisoning and the learning and memory,to provide the necessary theoretical and experimental basis. Objective:To explore the effect of arsenic trioxide on the adult neurogenesis of mice. Methods:The C57mice were treated with two different doses of arsenic trioxide by intraperitoneal injection.4weeks after administration, the mice received intraperitoneal injection of5-Bromo-2’-deoxyuridine and then were perfused with4%paraformeldhyde. The fixed brains were sliced by using cryotome. The sections were stained for mmunocytochemistry against BrdU to investigate quantificationally the proliferation of adult neural stem cells and survival of newly generated cells in the SGZ of the dentate gyrus. The density of BrdU-positive cells in the dentate gyrus were counted unter microscope and evaluated by SPSS17.0software. Results:In the group perfused24hours after BrdU injection, the mean number of control group(NS) is17.63, the low dose group(1mg/kg) is21.17, the high dose group(2mg/kg) is11.50; In the group perfused28days after BrdU injection, the mean number of control group(NS) is11.40, the low dose group(lmg/kg) is5.40, the high dose group(2mg/kg) is5.57.Conclusion:In a certain dose range, As2O3exposure induced cell proliferation, but impaired the survival of newly generated cells in the SGZ of the dentate gyrus in mice.