| Objective To investigate the expression of MRP4(multidrug resistance associated proteins) in osteosarcoma of extremities, analyze its correlation with clinical and histopathological characteristics, and further discuss its role in the prognosis of osteosarcoma.Methods Immunohistochemical stainning was performed to investigate the expression of MRP4in50cases of osteosarcoma which had complete follow-up data for more than three years.Among this group of patients,there were28males,22females;41stage ⅡB and9stage Ⅲ; with the median age of15.5(95%CI:15.73-20.93).Furthermore, its correlation with patients’ clinical manifestations, histopathological characteristics and its significance in prognosis of osteosarcoma was analyzed basing on the clinical follow-up data.Results This experiment analyzed the expression of MRP4in50cases of osteosarcoma. Compared with that in normal bone cells,MRP4was over-expressed in osteosarcoma cytoplasm (P=0.012). The expression of this marker has no significant difference in the Enneking clinical staging,gender,age,size and location of tumor(P>0.05). Kaplan-Meier analysis showed that in41cases of stage IIB patients,the disease free survival time in MRP4-over-expression patients was similar to that in low-expression/negative-expression patients (P=0.23),and in50patients(41cases of stage ⅡB and9cases of stage Ⅲ),the overall survival time in MRP4-over-expression patients was also similar to that in low-expression/negative-expression patients(P=0.40). In stage ⅡB patients, there was no significant difference between those who showed MRP4-over-expression and those who did not show MRP4-over-expression in the overall survival time (P>0.05).Conclusion MRP4expression in patients with osteosarcoma tissues was significantly higher than the normal bone tissue adjacent to the tumor, but MRP4is not a poor prognostic factor in patients with osteosarcoma,and the MRP4high expression in patients didn’t associated with disease free survival or overall survival. |
PDF Full Text Request