Expression And Clinic Significance Of SCD163and HO-1in Peripheral Blood Of Rheumatoid Arthritis

Objective: To detect concentration of sCD163and HO-1in peripheral blood, toanalyze the interclass difference and the correlations between the concentration and clinicalvariables in rheumatoid arthritis(RA). To research the value of sCD163and HO-1inthe pathogenesis of RA, the estimation of disease activity and prognosis.Methods: The fresh peripheral blood was collected from55RA patients and35healthy people. All the55RA patients were outpatient or inpatient from July2011toOctober2012of The First Affiliated Hospital of Soochow University. In RA patients，according to the disease activity was divided into two groups:29cases were remission，32cases were disease activity.38patients have joint deformity,17patients have not jointdeformity. Recorded rheumatoid factor (RF), anti-cyclic citrullinated peptide (CCP)antibody, swollen joint counts, tender joint counts, erythrocyte sedimentation rate (ESR),C-reactive protein (CRP), and related clinical indicators, such as the patient’s pain score(VAS), duration of morning stiffness, health questionnaire evaluation (HAQ), diseaseactivity score (DAS28). To detect the concentration of sCD163, HO-1in serum by ELISA.Results：(1)The concentration of sCD163in RA was remarkably higher than thecontrol group (P=0.006); Levels of sCD163in serum in disease activity were significantlyhigher than that in the NC group(p<0.05). Compared with remission group, levels ofsCD163in serum in disease activity were significantly increased, and the difference wasstatistically significant(P <0.05).(2)The concentration of HO-1in RA compared with theconcentration of HO-1in NC group was no significant difference(P=0.073). Levels ofHO-1in serum in disease activity were significantly higher than that in the NCgroup(p<0.05).Compared with remission group, levels of HO-1in disease activity groupsignificantly increased (P <0.05).(3) There were significantly difference between joint deformity group and joint not deformity group in disease activity of RA (P <0.005).Compared with joint not deformity group, concentration of HO-1in serum of jointdeformity group were significantly increased(P <0.05).The levels of sCD163in serumwere positively correlated with tender joint and swollen joint counts, DAS28score, CRP,RF, CCP.The levels of HO-1in serum were significantly correlated with DAS28scores.(4)The changed trend between the concentration of CD163and HO-1were the same.Correlation analysis shows there was a positive correlation between them(r=0.608, P<0.001).Conclusions：It is suggested that sCD163and HO-1in serum were involved in theonset and progression of RA. Concentration of sCD163and HO-1in serum could be as thejudgment of the disease activity indicators of RA. The sCD163and HO-1in serum werecorrelated with joint destruction, they may be played a role in the process of jointdestruction in RA, sCD163is related to, and interacts with HO-1, the two factors involvedin the occurrence and development of RA disease together.