Triptorelin Stimulation Test In Monitoring Triptorelin Therapy For Girls

Objective To determine the value of triptorelin （gonadotropin-releasing hormoneanalogue） stimulation test in monitoring triptorelin therapy for girls.Methods Thirty-one girls were recruited for this study, among whom26cases whosuffered with central precocious puberty were treated with triptorelin （diphereline）; and theother5cases of puberty （4cases with short stature of predicted adult height and1casewith idiopathic short stature） were treated with growth hormone co-treatment withindiphereline. Triptorelin stimulation test （decapeptyl,08:30a.m.,0.1mg, s.c.） wereperformed before the initiation of treatment as well as3months after the beginning oftherapy. The0min serum sample collected during the test was for diurnal serumspontaneous gonadotropin detection, and the sample collected at midnight before the testwas for nocturnal serum spontaneous gonadotropin detection. Luteinizing hormone （LH）and follicle-stimulating hormone （FSH） in serum were assayed byimmunochemiluminometric assays （ICMA）.Results Thirty in31cases had good clinical efficacy and the maximum of peak LH（PLH） after triptorelin therapy was1.68IU·L^-1. And the other1in31cases had poorclinical efficacy and her PLH after the therapy was3.81IU·L^-1. In these30cases withgood efficacy, PLH after and before triptorelin therapy were respectively （0.98±0.39）IU·L^-1and （26.11±16.59） IU·L^-1, and the former was significantly lower than the latter,P=0.000; and peak FSH （PFSH） after and before triptorelin therapy were respectively（1.65±0.85） IU·L^-1and （17.78±7.09） IU·L^-1, P=0.000. There was no overlap betweenminimum PLH before therapy and maximum PLH after therapy, so did between minimumPFSH before therapy and maximum PFSH after therapy. Diurnal spontaneous LH after andbefore triptorelin therapy were respectively （0.47±0.25） IU·L^-1and （1.88±3.24） IU·L^-1,P=0.000; and diurnal spontaneous FSH after and before the therapy were respectively（1.12±0.56） IU·L^-1and （5.28±4.49） IU·L^-1, P=0.000. In22/30cases with good efficacy, nocturnal spontaneous LH after the triptorelin therapy （0.48±0.27） IU·L^-1waslower than that before the therapy （4.55±4.54） IU·L^-1, P=0.000; nocturnal spontaneousFSH after the triptorelin therapy （1.16±0.47） IU·L^-1was lower than that before thetherapy （5.34±2.63） IU·L^-1, P=0.000.Conclusions A single serum sample for spontaneous gonadotropin determinationcan be used in preliminary assessment of the efficacy of triptorelin therapy for girls, andtriptorelin stimulation test （ICMA） may be of usefulness in monitoring triptorelin therapyfor girls.