Effect Of Selemum-Enriched Malt On Anti-Fatigue And Oxidantive Damage To The Mice Induced By Nicotine

ObjectivesTo research the effect and mechanism of selenium-enriched malt alleviating the physical fatigue in mice. To research the effect and mechanism of the SEM on anti-fatigue to the mice induced by nicotine. To research oxidantive damage of the SEM in mice. To research the SEM on the antagonism of oxidantive damage induced by nicotine.MethedsAccording to the priciple of weight banlanced, mice were randomly divided into12groups:①normal diet and saline,②normal diet and0.1mg/kg nicotine,③normal diet and0.5mg/kg nicotine,④normal diet and1.0mg/kg nicotine,⑤0.3mg/kg selenium-enriched malt(SEM) and saline,⑥0.3mg/kg SEM and saline,⑦0.1mg/kg nicotine and0.3mg/kg SEM,⑧0.1mg/kg nicotine and1.0mg/kg SEM,⑨0.5mg/kg nicotine and0.3mg/kg SEM,⑩0.5mg/kg nicotine and1.0mg/kg SEM,(11)1.0mg/kg nicotine and0.3mg/kg SEM,(12)0.5mg/kg nicotine and1.0mg/kg SEM,24rats in each group.,and weigh rats’body weights per5days. Mice in each group were killed at20days, Weight-bearing Swimming Time, Liver Glycogen, Blood Urea Nitrogen(BUN), Lactic Acid(LD), Adrenaline(AD), Dopamine(DA),5-hydroxy tryptamine(5-HT), Noradrenaline(NA), and the Superoxide Dismutase(SOD), Glutathione Peroxidase(GSH-Px), Malondiaidehyde(MDA), Total antioxidant capacity(T-AOC) of the heart, liver, kidney, brain, spleen and blood of the mice, were determined. Data were analyzed with normal ANOVA using the SPSS software(SPSS, version17.0).Results 1. The role and mechanism of the SEM alleviating physical fatigue in miceCompared with the control group, the level of BUN of medium dose SEM was significantly reduced(P<0.05), the level of liver glycigen was significantly increased(P<0.01). The weight-bearing swimming time and the level of liver glycigen of high dose SEM were significantly inreased(P<0.01), and the level of BUN and LD of Omin、20min after the swim were significantly reduced(P<0.05or P<0.01). The level of AD, DA,5-HT, NA of Omin、20min after the swim of medium and high dose SEM were higher than the control group, but without statistical significance.2. The effect and mechanism of the SEM on anti-fatigue to the mice induced by different doses of nicotineCompared with the control group, the weight-bearing swimming time of low-dose nicotine was significantly increased(P<0.05). the weight-bearing swimming time and the level of liver glycigen of0.1mg/kg nicotine and0.3mg/kg SEM and0.1mg/kg nicotine and1.0mg/kg SEM were significantly increased(P<0.05or P<0.01), and the level of BUN and LD of Omin-.20min after the swim were significantly reduced(P<0.05or P＜0.01).The level of AD, DA,5-HT, NA of Omn、20min after the swim of the above three gtoups were higher than the control group, but without statistical significance. Compared with the control group, the weight-bearing swimming time and the level of liver glycigen of medium dose nicotine and0.5mg/kg nicotine and0.3mg/kg SEM and0.5mg/kg nicotine and1.0mg/kg SEM were significantly increased(P<0.01), and the level of BUN and LD of Omin、20min after the swim were significantly reduced(P<0.05or P＜0.01).The level of AD, DA,5-HT, NA of Omin,20min after the swim of the above three gtoups were significantly higher than the control group(P<0.05or P<0.01). Compared with the control group, the weight-bearing swimming time and the level of LD of Omin、20min after the swim of high dose nicotine were higher, but without statistical significance, and the level of BUN is higher, the level of liver glycigen was lower. Compared with the control group, the level of liver glycigen of1.0mg/kg nicotine and1.0mg/kg SEM was significantly higher(P<0.01), the weight-bearing swimming time was longer, but without statistical significance, and the level of LD of Omin、20min after the swim were lower, but without statistical significance, but the level of BUN was higher.The level of AD, DA,5-HT, NA of Omin、20min after the swim of medium and1.0mg/kg nicotine and1.0mg/kg SEM were higher than the control group, but without statistical significance.3. Antioxidant effects of the SEM in miceCompared with the control group, the activities of the GSH-Px, SOD and T-AOC of the heart, liver, kidney, brain, spleen and blood of medium dose SEM were higher, and the level of the MDA was lower, but without statistical significance. Compared with the control group, the activities of the GSH-Px, SOD and T-AOC of high dose SEM were significantly higher(P<0.05or P＜0.01), and the level of the MDA was significantly lower(P<0.05or P<0.01), but the level of the MDA of spleen was lower, but without statistical significance.4. The effect of the SEM on oxidantive damage to the mice induced by different doses of nicitineCompared with the control group, the changes of the level of one or a few antioxidant indicators of the heart, liver, kidney, brain, spleen and blood of low dose nicotine were statistically significant. Compared with the low dose nicotine group, the activities of the GSH-Px, SOD and T-AOC of0.1mg/kg nicotine and1.0mg/kg SEM were significantly higher(P<0.05or P＜0.01), and the level of MDA was significantly lower (P<0.05or P<0.01).Compared with the control group, the activities of the GSH-Px, SOD and T-AOC of the heart, liver, kidney, brain, spleen and blood of medium dose SEM were significantly lower (P<0.05or P<0.01), and the level of the MDA was significantly higher(P<0.01). Compared with the medium dose nicotine group, the activities of the GSH-Px, SOD and T-AOC of0.5mg/kg nicotine and1.0mg/kg SEM were significantly higher(P<0.05or P<0.01), and the level of MDA was significantly lower (P<0.05or P＜0.01).Compared with the control group, the activities of the GSH-Px, SOD and T-AOC of the heart, liver, kidney, brain, spleen and blood of high dose SEM were significantly lower (P＜0.01), and the level of the MDA was significantly higher(P<0.01). Compared with the control group, the activities of the GSH-Px, SOD and T-AOC of the heart, liver, kidney, brain, spleen and blood of1.0mg/kg nicotine and0.3mg/kg SEM were significantly lower (P<0.05or P＜0.01), and the level of the MDA was significantly higher(P<0.05or P<0.01). Compared with the high dose nicotine group, the activities of the GSH-Px, SOD and T-AOC of1.0mg/kg nicotine and1.0mg/kg SEM were significantly higher(P<0.05or P＜0.01), and the level of MDA was significantly lower (P<0.05or P<0.01), but the activities of the GSH-Px, SOD of the of spleen was higher, but without statistical significance.Conclusion1. SEM and nicotine has no bad effect on the body weight, shape, activity and growth of mice.2. SEM and nicotine could significantly extend the weight-bearing swimming time, reduce the level of the BUN and LD of the movement, increase liver glycigen reserves, this could prove that SEM and nicotine alleviate the physical fatigue, and interaction between those is more effective.3. SEM increase the level of AD, DA,5-HT, NA of the movement is not obvious, so the mechanism of SEM alleviate physical fatigue is not to promote the secretin of AD, DA,5-HT, NA. Nicotine could significantly increase the level of AD, DA,5-HT, NA of the movement, so the mechanism of nicotine alleviate physical fatigue is that.4. SEM could significantly increase the activities of the GSH-Px, SOD and T-AOC, reduce the level of MDA, this could prove SEM to act the improvement of the antioxidant. Nicotine could significantly reduce the activities of the GSH-Px, SOD and T-AOC, increase the level of MDA, this could prove nicotine has a role to inceease the oxidantive damage.5. SEM coule significantly improve the abnormal changes of the activities of the GSH-Px, SOD, T-AOC and the MDA content, and tend to normal. It proves that SEM can inhibit the oxidantive damage induced by nicotine.