Neijing Regional Health Ideas Discussed And Enshi Authentic Chinese Herbal Medicine Of Model Organism Four Membrane Antioxidant Effect Research

ObjectiveThis study based on generalizations of Neijing regional health thought,interpretation of the regional difference of human body, disease, treatment,lifespan and subsequent formation has a direct impact, improved way of lifefor people, to improve environmental health, provides the basis for usingChinese drugs.This study follows the guidance of regional health preservation thoughttheory in neijing, and establish a pattern four biological membrane insectscreening platform for Chinese medicine, using enshi authentic medicinalmaterials, active ingredients Japanese ginseng Total Saponins of Panaxjaponicum (TSPJ, Total Saponins of Panax japonicus) direct interventionmodel, the method of observation on model biological antioxidant effect of theexperimental study, the preliminary discussion on traditional Chinesemedicine effective component intervention model biological oxidationmechanism.MethodMainly adopt the method of drugs directly added to culture mediaintervention four membrane, the water extraction liquid was observed directlyadd effects on four film insect life.Thermophilic four film the24hole plate of the worm in the sterile culture medium to join preparation good Japanese ginseng total saponins of16mg/ml(TSPJ) solution, inoculation amount of logarithmic phase thermophilic fourfilm culture, each concentration three parallel, setting up a blank control.Cultivation of30℃. Counting respectively in different temperature forsampling is the best concentration. After using the ultrasonic processor (phi)2broken cells, after four film worming cracking liquid for12000r. Centrifugal20min-1, take the supernatant. In ultraviolet spectrophotometer determinationkit biomedical engineering from Nanjing built the activity of superoxidedismutase (T-SOD). Using Q-PCR determination of GPX-4, TXNRD2genemRNA expression, each specimen for GPX-4mRNA, TXNRD2mRNA andinternal18s gene of fluorescence Q-PCR. According to four membrane inNCBI, TGD, TXNRD2mRNA GPX-4and18s internal gene sequences primerdesign.Result1. The four film bug of superoxide dismutase (T-SOD) activitydeterminationSuperoxide dismutase (SOD) is an important antioxidant enzymes inorganisms, oxidation and antioxidant on organism balance plays a vital role.Analyzed the Japanese ginseng total saponins (TSPJ) on the thermophilic fourinsect cell membrane SOD enzyme activity. Results show that thethermophilic four insect cell membrane T-SOD activity as the rho (TSPJ)increased (P<0.01).2. Different concentrations of Japanese ginseng total saponins affectmRNA, TXNRD2mRNA GPx-4GPx-4mRNA, TXNRD2mRNA genes are expressed in each group, afterlow concentration and high concentration of drug intervention. Compared withthe blank group, four membrane of thermophilic GPx-4mRNA,TXNRD2mRNA expression were significantly higher, with significant difference statistically significant (P<0.05)Conclusion1.The differences between the geographical environmental factorsdirectly affects the human body, disease, treatment, lifespan, and theformation of the climate, etc., comply with the regional health preservationthought in neijing, not only can effectively improve the human body health,the most conforms to the individual healthy lifestyle, and to the improvementof environmental health also has the very vital significance.2. Enshi, authentic Chinese herbal medicine-the main ingredient inJapanese ginseng Japanese ginseng total saponins has obvious anti-agingantioxidant effect, mechanism of action may be related to improving T-SODactivity, antioxidant GPX genes-4, TXNRD2expression.This studysummarized the "via" regional health care thought that geographicaldifferences directly affect the human body, disease, treatment, length of lifeand the formation of climate; regional health care thought from Heavencorresponding "point of view,"according to local conditions" for people’shealth care, anti-aging has a very important significance; conform to theregional health can not only effectively improve the physical health, thedevelopment of most individual healthy lifestyle, but also for the improvementof environmental health hasof great significance.