Studying On Faecal Protein, Amino Acid And Fatty Acid In Rotavirus Infection Diarrheal Infants

Rotavirus infection diarrhea was important cause about leading to acute diarrhea in globalinfants. Each year there were125million infants who were lead to diarrhea and900thousandinfants who were died because of RV infection around the world. This disease caused seriousinfluence for infants’health, growth and even lives.The incidences of RV infection diarrhea diseasein infant were influenced by infants’ years, feeding patterns and living areas. RV infection diarrheawas caused by damaging the intestinal structures and functions, making the intestinalmicroecological balance disorders, changing the balance of the host’s metabolisms and products,thus leading to the nutritional imbalance and appearing clinical symptoms about diarrhea. In thisstudy, we analysed faecal chemical compositions including protein, amino acid and fatty acid toreveale changes in human intestinal microecology, to explore the relationship between thechemical compositions and RV infection diarrhea disease and to provide scientific basis forpreventing and treating RV infection diarrheal disease.Faecal chemical compositions including protein, amino acid and fatty acid in the experimentalgroup consisting of15rotavirus infections diarrheal infants and control group consisting of15healthy infants which were diagnosed by Harbin city Children Hospital were as the object of thisstudy, and using questionnaires method was to collect the infants growth and disease informations;the total protein contents in the faeces were determined by coomassie brilliant blue method and thismethod was confirmed to be feasible through the stability test, the repeatability test, the precisiontest and the recovery test; the kinds and contents of faecal protein in RV infection diarrheal infantswere analysed by polyacrylamide gel electrophoresis methods; the kinds and levels of amino acidand fatty acid were analysed by chromatographic methods; lastly, the relationships between thedifferences of chemical compositions and RV infection diarrhea disease were determined.The results were as follows: the relative standard deviation values of the stability test, therepeatability test, the precision test and the recovery test in Coomassie brilliant blue method wereall less than5%, which showed the method could be used to determine total protein contents in thefaeces. The levels of total protein, polymeric-immunoglobulin receptor, lactoferrin, serum albumin,protein disulfide-isomerase and α1-antitrypsin were significant differences between experimentalgroup and control group (P<0.05). Compared with the control group, the levels of thepolymeric-immunoglobulin receptor and lactoferrin in rotavirus infections diarrheal infants werehigher, whereas the levels of total protein, protein disulfide-isomerase and α1-antitrypsin were lower. Serum albumin was only in the healthy infants. Other levels of protein ingredients were nodifferences between two groups (P>0.05). In RV infection diarrhea infants’faeces, the levels oftotal protein related to infants’ whether having dehydration and bowel sound frequency (P<0.05);the levels of polymeric-immunoglobulin receptor related to infants’age (P<0.05); the levels oflactoferrin related to age (P<0.05); the levels of α1-antitrypsin related to infants’birth place,feeding pattern, temperature, whether taking medicine during prgnancy and whether takingmedicine during lactation (P<0.05).The levels of lysine, phenylalanine, leucine, isoleucine, threonine, methionine, valine, alanine,proline, glycine, glutamic acid, arginine, serine, aspartic acid and histidine were significantdifferences between experimental group and control group (P<0.05). Compared with the controlgroup, they were lower in rotavirus infections diarrheal infants, whereas the levels of cystine andtyrosine were not (P>0.05). In RV infection diarrhea infants’faeces, the levels of threonine raltedto bowel sound frequency (P<0.05); the levels of methionine related to sex and bowel soundfrequency (P<0.05).The levels of C8:0, C11:0, C12:0, C14:0, C16:0, C18:1N9C, C18:1N9T, C18:2N6C, C20:2and C23:0were significant differences between experimental group and control group (P<0.05).Compared with the control group, the levels of C8:0, C11:0, C12:0, C14:0, C16:0and C18:1N9Cin rotavirus infections diarrheal infants were higher, whereas the levels of the C18:1N9T,C18:2N6C, C20:2and C23:0were lower. C8:0and C11:0were only in rotavirus infectionsdiarrheal infants. Other levels of fatty acids were no differences between two groups (P>0.05). InRV infection diarrhea infants’faeces, the levels of C11:0related to age(P<0.05); the levels ofC18:1N9T related to whether taking medicine during prgnancy and whether taking medicineduring lactation (P<0.05); the levels of C18:2N6C related to temperature (P<0.05); the levels ofC23:0related to feeding pattern (P<0.05).The results proved that there exited to be some relationships between the chemicalcompositions including partly protein, amino acid and fatty acid of faeces in rotavirus infectionsdiarrheal infants and risk factors of rotavirus infection diarrhea disease.