Efefcts Of Hematologic System And Bone Marrow Cells In Mice Induceed By Nano-Fe₃O₄Particles

Objective:In recent years, iron oxide nanoparticles are widely used in various fields,because of its good performance, applications in the biomedical field is a hotissue, in particular magnetic resonance contrast agent in a pharmaceutical carrier. Genetic engineering, hyperthermia is to focus on the development of nanotechnology, has sparked widespread concern on the safety of nanomaterials. Thisstudy used a non-coated magnetic nanoparticles of iron oxide （Fe₃O₄） a singletail vein injection of3days to observe changes in the mouse blood and bonemarrow cells indicators, the magnetic nanoparticles of iron oxide （Fe₃O₄） in the clinical safetyuse basis.Methods:Forty clean ICR mice （Number: males=females） were divided into two teams by gender.And each team was divided randomly into four groups as follows: high-dosage treatment group （40mg/kg，1/4LD50）, middle-dosage treatment group （10mg/kg，1/16LD50）, low-dosage treatment group （2.5mg/kg，1/64LD50）, and pure water negative control group. Nano-Fe₃O₄was injected through tail vein of mice once.The injectional volumn was10ml/kg and the speed was0.02ml/s. Observed after3days in mice peripheral blood cells and their classification, red blood cells, serum, bone marrow cells from oxidative damage （SOD,MDA, GSH-Px）, mitochondrial membrane potential of bone marrow cells, bonemarrow cell cycle and apoptosis of bone marrow cells exposed to the DNA damage and other indicators.Results:1.Changes of peripheral blood cells of miceThe WBC female mice each dose group were significantly higher （P<0.05）, male mice in the lower dose group, high dose group increased （P<0.05）, LYM%female mice in the high dose group increased P<0.05)high dose group ofmale mice decreased （P<0.05）, MID%female mice each dose group were lower （P<0.05）, Gran%of female mice in each dose group were significantly increased （P<0.05）. PLT female mice high-dose group were significantly higher （P<0.05）, each dose group of female mice were significantly increased （P<0.05） in male mice, the low-dose group （P<0.05） lower, MPV, PDW, PCT female mice of each dose group with the control group showed no significant difference（P>0.05）. RBC, HGB, HCT, MCH female mice of each dose group were lower (P<0.05in each dose group and the control group of male mice was no significant difference （P>0.05）, MCHC, RDW-CV, RDW-SD male and female mice and the control group showed no difference （P>0.05）.2. Changes of MDA、SOD、GSH-Px in mouse peripheral blood erythrocyteMDA of high-dose group were significantly higher in male and female mice （P<0.05）, female mice in the high dose group was significantly higher （P<0.05）, no significant differencebetween each dose group of male mice （P>0.05）.High doses of SOD activity in male and female mice was significantly lower（P<0.05）, SOD group of female mice in doses higher than the low-dose groupand the control group （P<0.05）, no significant difference between the low-dose group and control group （P>0.05）. GSH-Px activity in female mice of middle and high dose group lower than the control group （P<0.05）, GSH-Px was significantly lower in each dose group of male mice（P<0.05）.3. Changes of MDA、SOD、GSH-Px in mouse peripheral blood serumMDA of high-dose group were significantly higher in male and female mice （P<0.05）, male mice increase with the dose increased （P<0.05）. SOD activity of high-dose group was significantly lower in male and female mice （P<0.05）, female mice increase with the dose decreased （P<0.05）. GSH-Px activity inmale and female mice, the middle dose group was significantly higher than that in the control group （P<0.05）, but the high-dose group was significantly low er （P<0.05）.4. Changes of mitochondrial membrane potentials in mouse bone marrow cellsEach dose group of bone marrow cells line the three-dimensional membrane potential was significantly lower than the control group （P<0.05）, but no significant difference between each dose group（P>0.05）.5. DNA damage of mouse bone marrow cellsEach dose group of0damage lower than the control group in male and female mice bone marrow cells （P<0.05）, and the proportion of injury decreasedwith the dose increased. Each dose group I injury is significantly higher thanthat in the control group （P<0.05）, and with the dose gradually increased （P<0.05） increased the extent of damage.≥II injury only appear in the high-dosegroup.6. Changes of cycle and apoptosis in mouse bone marrow cellG0/G1phase increase in low-dose group of female mice bone marrow cells, each dose group lower of male mice （P<0.05）. S phase of each dose groupwere lower in female mice, each dose group of male mice increased （P<0.05）.G2/M phase、apoptosis rate were increased of female mice （P<0.05）.7. Changes of MDA、SOD、GSH-Px in mouse bone marrow cellsMDA between the control group showed no significant difference of female mice bone marrow cells （P>0.05）, the high-dose group was significantly lower of male mice （P<0.05）. SOD, GSH-Px activity of the high-dose group wassignificantly lower in male and female mice （P<0.05）.Conclusion:1.In this condition，The nano-Fe₃O₄can change the peripheral blood of mice in the WBC, RBC, PLT count their classification, can make red blood cells, increased serum MDA, SOD, GSH-Px activity is reduced, causing the red bloodcells, serum lipid peroxidation in mice after three days.2.In this condition，The nano-Fe₃O₄can reduce MDA, SOD, GSH-Px activity decreased oxidative damage caused by bone marrow cells; can cause decline inmitochondrial membrane potential; bone marrow cells can cause DNA damage; affect the bone marrow cell cycle progression, G2/M phaseblock, resulting in apoptosis of bone marrow cells.