The Study Of Liver And Kidney Function And Oxidative Stress In Fluorosis Rats

Fluorine as a potentially toxic trace elements, in addition to significant damageto the skeletal system, the of phrenology system also have a serious impact.Especially the liver and kidneys. The important place of the liver is thedetoxification of the body, the kidney is an important way to fluoride excretion,and therefore they are the vital organs of the toxic effects of fluoride. There havebeen some reports about fluoride liver and kidney dysfunction, but because of thedifferent experimental conditions and applied factors, and the experimental resultsare different. The mechanism of liver and kidney damage caused by fluoride is notclear. Most scholars believe that oxidative stress, but inconclusive.In this study, the conventional feed and the partial eclipse fed, drinking wateradded fluoride copy rat model of fluorosis, and observe the changes of the differentexperimental period fluorosis rat liver and kidney function and oxidative stresssystem, to investigate the damage of oxidative stress and liver and kidney functionrelationship may exist, trying to prove that certain time effect of fluoride on rat liverfunction and kidney toxicity, this time may be related to the anti-oxidative stressfunction. The first imposition of the natural antioxidants soybean the isoflavones(Soybeen isoflaono SIF) to observe the reduction of fluorine caused liver andkidney toxicity and oxidative stress effect, to provide scientific clues for theprevention and treatment of endemic fluorosis.Methods:Firstly, the method of drinking water with fluoride(F-100mg/L) copyfluorosisanimal model in rats. Then dividing300Wistar into three groups forexperiments.Each experiment is divided into five groups, specific as follows: Regular feed control and adding fluorine groups, The partial feed control and addingfluorine groups, The partial feed plus fluorine and SIF group; these groups weredrinking water fluoride12weeks,15and35weeks in experiment one, two, three.The observation projects including rat body weight, the fluorine spot tooth appearstime and general condition changes，the liver and kidney function in rats(using thebiochemical automatic analyzer detected the fluorosis rats the changes in serumaspartate aminotransferase(AST)，alanine aminotransferase(ALT)，Lactatedehydrogenase (LDH)，Alkaline phosphatase (ALP)，blood urea nitrogen(BUN)，creatinine(CRE)and Uric Acid(UA) activities; and the resistance to oxidative stresssystem change use the application of biochemical reagent kit measure liverhomogenate of catalase(CAT)， superoxide(SOD)， glutathione peroxidase(GSH-PX)and purities of serum lipid peroxide benzene dialdehyde.Result：1. The weight and survival status of fluorosis rats in12weeks, the liverfunction renal function and oxidation stress in good condition but to the weight lossis more noticeable;2. By15weeks, the fluorosis rats weight loss significantly, and renal functionindexes and oxidative stress indicators of a significant change, add the SIFcanobviously change the symptoms;3. By35weeks, there was so little difference in the body weight in each groupof rats. The liver renal function appeared significant change. The oxidative stresschange significantly, add SIF can obviously change the symptoms.4. The result of serum uric acid showed, fluoridated week of12, there wasnosignificant differences in each groups; by week15, in addition to the partialeclipse add fluoride group was significantly lower than the control group,nosignificant difference between other groups, by week35, each fluoride group werehigher than the corresponding control group, the partial eclipse fluoridate group issignificantly higher than the control group to join SIF canadjust the change. Conclusion:1. Add fluoride12and15weeks, the fluoride group rats weight compared withits corresponding control group decreased obviously, by35week, there was nodifference between the each weight, it showed the general condition of rats tofluorine toxicity has certain adaptability;2. The Liver kidney function of fluorosis rats change with time effect that istaken fluorine initial change is not obvious,they would appear more obviouschange over time, and time extension for fluorine were more obvious,but the renaltoxicity of fluorine effect appeared earlier and a longer duration;3. The oxidative stress test results showed that in early time, fluorosis ratsoxidation stress function was improved remarkably, that may cause fluorine intakeof early kidney function was one of the important reasons for the change is notobvious; but with longer duration of fluorosis, lipid peroxides increasedsignificantly,their liver and kidney function damage symptoms began to emergeandgradually increase;4. Isoflavoneas a kind of natural antioxidants, which can obviously theoxidative stress injury effect of antagonist fluorine, has the possibility of preventionand treatment of endemic fluorosis;5. The adding fluoride group of three groups of experiments of liver and kidneyfunction, and oxidative stress state changes from partial fluorine groups wassignificantly reduced.The results for the calcium nutrition state in fluorosis providesfurther evidence of the important position.