| ObjectiveExplore the influence of new sevoflurane inhalation anesthetics to thepostoperative cognitive function of elderly diabetes rats and the centralneurotransmitter acetylcholine content in hippocampus, and thus preliminarily revealthe mechanism of sevoflurane influence of postoperative cognitive impairment.Methods1. Establishment of elderly diabetic rat model: age18-20months SD100rats,body weight450-500g, female in half.80rats which have good memories testdetermined by Morris Water Maze were randomly divided into control group (group A,n=10) and model group (group B, n=70). Group A was the general diet, group B was withhigh fat and sugar feed+5%glucose water feeding, making the insulin resistance. After2weeks, continuous intravenous injection of30mg/kg STZ for3days, induce persistenthyperglycemia, and then the rats were continued to observe for8weeks. Every2weeksrat’s body weight, blood glucose and serum insulin were determined.2. After produced in the elderly diabetic rat model, rats were randomly dividedinto control group (group C), inhalation of2%sevoflurane for2h after1d group and7d, inhalation of isoflurane for2h after1d and7d group:1days without inhalationgroup (Group S1, C),1d with2%sevoflurane inhalation group (S1, S),1d2%isoflurane inhalation group (S1, I),7days without inhalation group (S7, C),7days2%sevoflurane inhalation group (S7, S),7days2%isoflurane inhalation group (S7,I). Rats in each group were in the right hind the bottom row of0.25%bupivacaineinfiltration anesthesia, the control group (S1, C) and control group (S7, C), with localinfiltration anesthesia in Brennan rat plantar incision, suction alkyl group (S1, S, S1, I,S7, S, S7, I) in sevoflurane or isoflurane combined with local infiltration anesthesiain case of Brennan rat plantar incision operation. The rats, which inhaled sevofluraneand isoflurane anesthesia after first days and seventh days respectively, began theMorris water maze test,4times a day,5days of continuous measurement. After Morris water maze test, the mice were immediately guillotined, isolated thehippocampus, using ELISA method for the determination of hippocampalacetylcholine content, compared with the control group.Results1. High-sucrose and high-fat feed with5%glucose water and continuouslow-dose STZ can build the ideal type2diabetes rat model, which is similar with thehuman type2diabetes, and this model is shortened established relatively.2. After1d inhaled2%sevoflurane, the experimental results of elderly diabeticrats positioning navigation and space exploration are lower than those in controlgroup (P <0.05). After1d inhaled2%Isoflurane, the experimental results of ageddiabetic rats positioning navigation and space exploration were also lower than thosein control group (P <0.05). Tn brain acetylcholine content in the hippocampus alsodecreased in these two groups (P <0.01), and the acetylcholine content in theisoflurane group decreased more obviously than the inhalation of sevoflurane.Inhalation of sevoflurane7days later, rats positioning navigation and spaceexploration experiment results and acetylcholine content are lower than in controlgroup, but there is no statistical significance (P>0.05). Inhalation of isoflurane in ratsafter7days, the positioning navigation and space experiment results andacetylcholine content had increasing trend, but compared to the control group, wasstatistically significant (P <0.05).Conclusion1. This experiment successful builded Type2diabetes rats model. This model ismore similar to human type2diabetes. It provides a good animal model for furtherexperimental study.2. Inhalation of sevoflurane can make aged diabetic rats in the earlypostoperative cognitive function transient decline, but compared with positive controlmedicine isoflurane, early postoperative cognitive function recovered faster, and thereis no long-term postoperative cognitive function of the adverse impact. Sevoflurane islikely due to decreased neurotransmitter acetylcholine which affect the ability oflearning and memory. |
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