Studies On Screening The Superior Kinds Of Schtsandrae Chinensis Fatctus And Preparation Of Lignan Components

Schisandra chinensis(Turcz.)Baill, commonly known as “Fructus schisandrea”.Mainly distributed in the northern China. The fruit were used for medicine,Schisandrae chinensis fructus generally have sour, sweet, bitter, piquancy, salty.Schisandrae chinensis fructus has a number of pharmacological effects, and a varietyof active ingredients, and thus has a wide range of pharmaceutical market prospects.In addition to medicinal Schisandrae chinensis fructus also used for drinks、food,therefore requires a lot of Schisandrae chinensis fructus resources. Lignans are themost important effective constituent in the Schisandrae chinensis fructus, have theactions of protect liver, anti-viral, anti-tumor and anti-aging. Schisandrae chinensisfructus is the object of study, the main findings are as follows:1. Screening the superior kinds of Schisandrae chinensis fructus by using patternrecognition method. Ultra performance liquid chromatography (UPLC) was used todetermine the content of lignans of Schisandrae chinensis fructus, samples were30that with different varieties from Tonghua area. The antioxidant activity of thesesamples was also studied. In order to screen the superior kinds of these samples,principal component analysis and cluster analysis were used. Both principalcomponent analysis and cluster analysis divided the origin samples into threecategories. The correct rate for screening the superior kinds of Schisandrae chinensisfructus via stepwise discriminstion analysis was90%.2. According to the orthogonal experimental design, the optimum extractionconditions of lignans from the Schisandrae chinensis fructus was showed as following:extracting solvent was80%ethanol, solid-liquid ratio was1:10, extracting time was60minutes. D101macroporous resins were used to pure lignans, the best purificationprocess for obtaining total lignans content was showed as following: in order toobtain lignans with the density of1.01mg/mL, the maximum loading amount is6times to the column volume, the flow rate is1.0mL/min, distilled water and80%alcohol takes9and7times to the column volume, respectively. With this advancedpurification process, the total lignans can reach64.78%, indicating that this method is able to achieve the purpose of the purification lignans from Schisandrae chinensisfructus.3. The first use of high-speed countercurrent chromatography (HSCCC) toisolate and separate schisandrin、schisandrol B、schisantherin A and deoxyschizandrin,the solvent system composed of n-hexane-ethyl acetate-methanol-water (9:1:5:5), theupper organic phase system as the stationary phase and the lower phase system as themobile phase, flow rate is2.0mL/min, speed is900r/min. Run150minuties use lowerphase of n-hexane-ethyl acetate-methanol-water (9:1:9:5) as the mobile phase. UPLCwas used to determine the content of schisandrin、schisandrol B、schisantherin A anddeoxyschizandrin were98.74%、99.53%、94.23%and98.68%.4. Estabish the mouse model of alcohol liver injury and to investigate the effectsof the Schisandrae chinensis fructus ultrasound extract. Schisandrae chinensis fructusultrasound extract can significantly reduce the activity of ALT and AST, andsignificantly enhance the activity of ADH. The results suggest that Schisandraechinensis fructus can dispel the effects of alcohol and protect liver.