Research Of The Oxidative Stress Injury Of Pesticide Atrazine On Ovarian Tissue In Rat

Ovarian cancer is one of the most common malignant tumor of female reproductivesystem.Because of the deep position of ovarian in the abdominal cavity,it is hard to diagnosisthis disease, and usually discovered late.Therefore, the lack of effective early diagnosismethods, the mortality in the female reproductive system tumors is in the first.The5yearsurvival rate of patients with ovarian cancer, early70%~90%, late for only20%.Epidemiological data show the number of pregnancy, breast-feeding duration and oralcontraceptives can reduce the risk of ovarian cancer, and the mechanism is closely related togonadotropin and the corresponding receptor expression.To understand the mechanism ofoccurrence of ovarian cancer and how to prevent ovarian cancer, has become a globalproblem to be solved.Many studies have shown that, the occurrence of some cancers is closely related tooxidative stress and DNA damage.Atrazine, is now a global most widely used herbicide andone of the largest.In environment, it can exist for a long time, and can accumulate in animalbody, having multiple system toxicity, has been more and more attention.However, researchon ATR damage of ovarian tissue is still very little, so the research on the influence of ATRon the ovary is helpful to understand the mechanism of damage to the ovarian tissue, is ofgreat significance for the prevention and treatment of atrazine cause ovarian damage, andcan predict the possible causes of ovarian cancer occur in some extent. In this study, Wistar rats treated with ATR, were randomly divided into control group,low, middle, high dose group4, administrated with corn oil,5mg.·kg-1,25mg·kg-1and125mg·kg-1atrazine,1times a day, continuous administration of28days.Using HE staining toobserve the changes of ovarian tissue. ovarian tissue MDA, NO content and SOD, CAT,GSH-PX activity were detected by biochemical method, immunohistochemistry staining wasused to detect Nrf2intracellular localization, western blot was used to detect the expressionof Nrf2, KEAP1, HO1, NQO1, ATM, p-ATM, CHEK1, p-CHEK1, P53and p-P53in ovariantissue homogenate.Results show that, from the expression of tissue morphology, tissueenzyme activity to specific gene protein, occurring of oxidative stress and DNA damage inovarian tissue.In this experiment, ATR was successfully induced ovarian tissue oxidative damage, thestudy found different effects of each dose group ATR on ovarian morphology, enzyme andDNA damage.To study the effect of Nrf2and ATM pathway in atrazine exposure process,will contribute to the understanding of mechanism of atrazine ovarian damage, is of greatsignificance for the prevention and treatment of ovarian damage caused by atrazine.