The Effect Of Mesenchymal Stem Cells To Treat Acute Liver Failure

Fulminant hepatic failure (FHF) is an acute severe liver failure, dangerous disease,theprognosis is poor, is still one of most difficult to treat in the medicine. In ourcountrythe,A typical representative of the common is Severe viral hepatitis，the mortalityrate up to90%. The treatment of liver failure Include medical therapy, artificial livertreatment and livertransplantation. It does’t have Effective medicine to medical treatment,and efficacy is limited.Liver transplantation is limited due to the shortage of donor source,immune rejection and high transplant costs.Artificial liver support its efficacy and safetyhas been a certain degree of certainty,but the source of liver cells difficult to makebioartificial liver research into a trough at home and abroad.In recent years, the rapid development of stem cell research brings new hope to solvethe problem of the source of the cells of the liver failure treatment. MSCs initially found inthe bone marrow,Because of its differentiation potential of hematopoietic support andpromote stem cell implantation, immune regulation and increasing attentions. Havebecome an increasing concern. MSCs Has been used in a variety of diseases, tissue repairin the Treatment and Research. So the use of cell transplantation to repair, treatment ofacute and chronic liver failure patients to help patients waiting for liver transplantation ordirect repair failure, liverpassed the critical stage is an ideal method.The subject is based on MSCs differentiation potential and ability to differentiate intohepatocytes, immunomodulatory function in recent years proved as an important clue, theimmune pathogenesis of liver failure as the basis,to Carry out the experimentaltwo-dimensional co-cultured liver cells and MSCs,Design the study based on theestablishment of a reasonable cell damage and animal liver failure model to stduy Theeffect of supernatant liquid form co-culture of MSCs with hepatocytes on L02in vitro andthe treatment of liver failure animals.Our results showed that the supernatant liquid ofco-cultivation of hepatoctyes and MSCs.as well as the cultred hepatoctyes and MSCs canpromote the cell viability and proliferation of L02cells in vitro. Both the cell actively andthe total protein synthesis of cultured L02cells increased under the supernatant liquid. Theamount of leakage of AST、LDH and cell apoptosis of damaged L02cells by alcohol werelowered on the effect of culture supernatant liquid. Among the three supernatant liquids,the effect of the supernatant liquid of co-culture was most remarkable (P<0.05).At thesame time, the results of our animal experiments showed the MSCs-liver cell co-culture supernatant can effectively reduce the mortality of the KM mice,improve the liver functionof rats.effectively.The KM mice mortality rate of Co-cultured group was30%, significantlybetter the group which was cultured alone, while the control group of mice with a mortalityrate of80%. The different composition of the cell culture supernatant was spreliminaryidentified by antibody array.Our results showed that IL-1,IL-6,IL-8and other cytokinestheof supernatant liquid of co-cultivation of hepatoctyes and MSCs were significantly higherthan the amount of cytokines in the supernatant separately cultured hepatoctyes and MSCscultured.