Protective Effect Of Lithium Chloride On The Apoptosis And Nuclear AIF Translocation Of SH-SY5Y Cells Induced By Oxygen And Glucose Deprivated

ObjectiveTo study the protective effects of the different concentrations of lithium onthe SH-SY5Y cells oxygen and glucose deprivation model, then to determinethe effective concentration, and to explore the effect of nuclear AIFtranslocation.MethodsDifferent concentrations (0mM/L,0.2mM/L,0.5mM/L,1.0mM/L,1.5mM/L,2.0mM/L) of lithium chloride preconditioned the oxygen and glucose deprivationmodel of SH-SY5Y cells and were divided into6groups (M1~6) then they wereused to observe the protective effects on cells. MTT staining were used todescribe the effects of the drugs and choose the best time point of oxygen andglucose deprivation and protective concentration of lithium chloride. SH-SY5Ycells passaged by2days were divided into three groups(control group, oxygenand glucose deprivation group and lithium chloride group). The cell apoptoticrate was analyzed by flow cytometry after Annexin V/PI double staining. AIFprotein was detected by immunofluorescence staining and western blot.ResultsMTT showed that different concentrations (1.0mM/L,1.5mM/L,2.0mM/L) oflithium chloride had stronger protective effects on the oxygen and glucose deprivation model of SH-SY5Y cells than the other concentrations(P<0.05), and1mM/L lithium chloride was the effective dose for cell protection; but increasingthe concentration of lithium chloride, the protective effects have not beenstrengthened (P>0.05). Compared to the oxygen and glucose deprivation group,Annexin V and PI staining, flow cytometry showed that the apoptosis wasdecreased significantly in cells treated with lithium chloride(p<0.01).Immunofluorescence staining and western blot suggested that AIF proteinreduced in nuclear treated with lithium chloride (p<0.01).ConclusionsLithium has strong protective effect on the oxygen and glucose deprivationmodel of SH-SY5Y cells,1mM/L lithium chloride, the effective dose for cellprotection, one of whose mechanisms may be the inhibition of nuclear AIFtranslocation.