| Objective1.To study the effect of human bone marrow-derived mesenchymal stemcells (hMSCs) transplantation through trachea on the major inflammatorycytokines and lung tissue injury in rats with hot smoke inhalation injury.2.To study the effect of human bone marrow-derived mesenchymal stemcells (hMSCs) transplantation on the major inflammatory cytokines and lungtissue injury in rats with hot smoke inhalation injury.3.To discuss the effect of stem cells prevention by different transplantationways.Methods1.Design and manufacture an equipment to simulate hot smoke environmentat the scene of the fire. Fifty-six healty Wistar rats were randomly divided intocontrol group (C group, n=8), hot smoke inhalation injury group(SQ group, n=24)and MSCs engraftment by trachea after hot smoke inhalation injury group (MQgroup, n=24). The latter two groups were subdivided into2，4，8hours afterinjury subgroups, with8rats in each group. The rabbits were killed at scheduledtime points,control group after8h,the smoke inhalation injury group at2h,4hand8h after injury.The levels of TNF-α、IL-6and IL-10in blood serum andbronchoalveolar lavage fluid (BALF) were measured by enzyme-linkedimmunosorbent assay (ELISA). The lung tissue were obtained to measure andcalculate lung water mass fraction, the other lung tissue was harvested for morphology and histopathology observation.2. Forty-eight healty Wistar rats were randomly divided into hot smokeinhalation injury group(SV group, n=24) and MSCs engraftment by caudal veinafter hot smoke inhalation injury group (MV group, n=24). Then the two groupswere subdivided into2，4，8hours after injury subgroups, with8rats in eachgroup. The levels of TNF-α、IL-6and IL-10in blood serum and bronchoalveolarlavage fluid (BALF) were measured by enzyme-linked immunosorbent assay(ELISA). The lung tissue were obtained to measure and calculate lung watermass fraction, the other lung tissue was harvested for morphology andhistopathology observation.Results1. Compared with C group,concent of pro-inflammatory andanti-inflammatory cytokines in blood serum at each time point in SQ group wereincreased obviously. The concent of pro-inflammatory cytokines in BALF ateach time point in SQ group was significantly higher than those in C group, andthat of anti-inflammatory cytokines showed no significant changes. Comparedwith the SQ group, concent of pro-inflammatory cytokines in blood serum in MQgroup a4hours and8hours was decreased markedly, and that ofanti-inflammatory cytokines was inceased significantly.pro-inflammatory andanti-inflammatory cytokines at2hours showed no significant changes. Concentof pro-inflammatory cytokines at each time point in MQ group was deseasedsignificantly when compared with SQ group in BALF, with oniy anti-inflammatorycytokine at4hours and8hours was increased significantly. Lung water massfraction in MQ group was significantly lower than that in SQ group. Comparedwith that in C group, lung water mass fraction in SQ and MQ groups wereinceased significantly. Lung tissue injury in MQ group was alleviated obviouslyas compared with that in SQ group.2. Compared with C group,concent of pro-inflammatory and anti-inflammatory cytokines in blood serum at each time point in SV group wereincreased obviously. The concent of pro-inflammatory cytokines in BALF ateach time point in SV group was significantly higher than those in C group, andthat of anti-inflammatory cytokines showed no significant changes. Comparedwith the SV group, concent of pro-inflammatory cytokines in blood serum in MVgroup was decreased markedly, and that of anti-inflammatory cytokines wasinceased significantly.Concent of pro-inflammatory cytokines at each time pointin MV group was deseased significantly when compared with SV group in BALF,with oniy anti-inflammatory cytokine at4hours and8hours was increasedsignificantly. Lung water mass fraction in MV group was significantly lower thanthat in SV group. Compared with that in C group, lung water mass fraction in SVand MV groups were inceased significantly. Lung tissue injury in MV group wasalleviated obviously as compared with that in SV group.Conclusion1. hMSCs engraftment by trachea confers some protective effect on hotsmoke inhalation injury.2.hMSCs engraftment should desease pro-inflammatory cytokines andincease anti-inflammatory cytokines in the early stages of hot smoke inhalationinjury, therefore, ameliorates inflammatory responses,which confers protectiveeffect on hot smoke inhalation injury.3.stem cells both can protect the injury lung by the two transplatation ways. |
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