Experimental Study Of Vacuum Aspiration To Promote The Regeneration Of Rat Sciatic

ObjectiveTo study whether vacuum aspiration conduce to the regeneration of ratsciatic nerve or not, and which value of vacuum aspiration is more effective.MethodsStudyⅠ. Thirty adult S-D rats, weighted250-300g, were distributed to threegroups randomly. We did chop amputation in both sides of sciatic nerves in allrats. The proximal stumps of right side of sciatic nerves were sucked by vacuumaspiration and the left side ones were given no treatments as contrast. Thevalue of vacuum aspiration was different in three groups:50mmHg in group A,100mmHg in group B and150mmHg in group C.StudyⅡ. Other thirty adult S-D rats, weighted250-300g, were also givenchop amputation in both sides of sciatic nerves. The right side of sciatic nerveswere experimental ones and the left ones were as contrast. Then stumps of bothside of sciatic nerves were sutured. Nerves in experimental side were insertedin some special silica gel tubes, which connected to vacuum aspiration andmade the stomas of the nerves sucked by vacuum aspiration. Nothing was donein contrast side.ResultsOne month after operation, in study Ⅰ, regenerations were observedobviously in the nerve stumps treated by vacuum aspiration, which hadstatistical difference compared with contrast groups. Among the three groups A B C, regenerate nerves in group B were longer and had more axons than othersin group A and C (P <0.01). In studyⅡ, restoration at stomas of nerves inexperimental side was better than ones in the contrast side.ConclusionsVacuum aspiration can promote regeneration of sciatic nerve, itsmechanism may not be mechanical extension but regeneration of new axons.The value of vacuum aspiration at100mmHg shows more effective to nerveregeneration.