| ObjectiveTo investigate and detect the effects of estrogen on bone mesenchymalstem cells osteogenesis differentiation of mouse, and find out the relationshipswith mir-26a.MethodsBone mesenchymal stem cells were isolated from C57mouse which is4-8weeks,femal,healthy and purified by the ability to adhere to the culturalplastic and then osteogenic in different consentration estrogen(0、10-6、10-7、10-8、10-9、10-10mol/L) in vitro. The proliferation ability was measured throughMTT method. The effects of estrogen on BMSCs induced osteogenesisdifferentiation were determined by alizarin red staining to analysis calcium saltdeposits and RT-PCR，Western blot to analysis runx2,sp7after induced2weeks.The expression of miR-26a measured through RT-PCR.ResultsCell surface expression of CD molecules consistent with reported before，and with both osteogenic and adipogenic differentiation ability. The difference ofdifferent concentrations of estrogen on cell proliferation rates has no obviouslystatistics sense,10-8mol/L group is fastest,10-9followed. Osteogenicdifferentiation capacity of each group compared with the no estrogen groupwere significantly increased (P <0.05),10-9group is strongest (P<0.01), both the molecular level of ocn, runx2and the protein detcte of sp7,runx2are higher thanother groups. The expression of miR-26a which obtain at different time points inthe normal osteogenic induction increase with the extension of the inductiontime measured, which receive in different density groups is contrary with ocnand runx2，10-9group is decrease significantly.ConclusionsThe osteogenesis differentiation ability of10-9mol/L group is obviouslyhigher than other groups, both gene and protein level. RT-PCR analysisindicated that the expression of miR-26a changed after stimulated by estrogen.The expression of miR-26a is down-regulation during the osteogenic stronglythat maybe demonstrate the estrogen could affect the miR-26a expression. |
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