Proliferation And Apoptosis Of Glioma U251Cells Induced By SiRNA Against APE1

ObjectiveTo explore the effect of proliferation and apoptosis of glioma cells inducedby siRNA against APE1.MethodsDivide the experiment into APE1siRNA group (transfected with APE1siRNAexpression plasmid),control group(transfeced with empty plasmid) and negativegroup (not transfected with any plasmid).Transfected into human glioma cellsU251cell lines in accordance with the grouping.The expression of APE1proteinwas studied by western blotting.The proliferation and apoptosis of glioma cellswere studied by MTT assay and AO/EB double flourescent dye staining,drowthe cell survival curve with MTT.ResultsWestern blotting shows that the APE1expression of APE1siRNAgroup(0.223±0.017) is lower significally than the control group (0.836±0.048)and the negtive group(0.914±0.076).MTT assay shows the growth of cells inthe APE1siRNA group(0.452±0.006，0.513±0.021，0.673±0.055，0.883±0.077，1.125±0.096) is inhibited significally than control group(0.451±0.006，0.723±0.002，1.092±0.042，1.521±0.060，1.734±0.034)and negativegroup(0.452±0.007，0.710±0.015，1.102±0.066，1.543±0.063，1.781±0.066).It is found that the apoptosis rate of APE1siRNA (31%)is higher thancontrol group(7%) and negative group (3%) by AO/EB double fluorescent dye staining and this conparison have significant difference.But the control groupcompared with the negative group has no significant difference.ConclusionsIt can inhibit the human glioma cells proliferation and promote theirapoptosis after the transfection of APE1siRNA.