Effect Evaluation That Arsenic Trioxide Remedy9L Glioma Of Rat’s Brain

BackgroundGlioma is a common tumor of the nervous system,because of high degree of malignancy it is a serious threat to human health.Its treatment has been a medical problem. At present operation is main treatment of the glioma,combining radiotherapy with chemotherapy. It is difficult to be thoroughly resection of the glioma,the radiotherapy effect is not obvious, chemotherapy is main therapeutics for gliomas. Because blood vessel of tumor is damaged, Intratumoral administration can increase medication options. Therefore, it is necessary to further validate its efficacy in vivo animal.ObjectiveTo observe the efficacy which arsenic trioxide treat glioma in model rat,and to provide experimental date for glioma treatment.Method1.The rat glioma model established we implant9L cell in striatum of rat by microinjection, some of rats was executed after7days and get out the brain of rats in order to confirm9L cell injected into the brain, the rest of rats weight, diet and life span were recorded until they died. The rats were kille not to exceed1weeks, and get out the brain, staining with HE.2.Curative effect observation about arsenic trioxide treat glioma.control group(without implanting9L cell injecting the same amount of saline), tumour group(implanting9L cell injecting the same amount of saline), low dose group (5μmol/L), middle dose group (10μmol/L), high dose group (20μmol/L),which was implanted9L cell and treated by arsenic trioxide12days after.the same time some of rats was executed and get out the brain of rats, the rest of rats weight, diet and life span were recorded until they died. comparing the groups of tumor tissue cell morphology and proliferating cell nuclear antigen (PCNA) changes.Results1.Curative effect observation about arsenic trioxide treat glioma The effect is not obvious for animation weight life cycle and low dose group. High concentration group (20μmol/L) treatment effect is no superior in concentration group (10μmol/L), but the side effects of more obvious. concentration group (10μmol/L) tumors was significantly smaller than the untreated group, tumor induced by inhibition of weight loss, prolo-nged survival. after treatment, tumor boundaries more clearly with normal tissue; prolifer-ating cell nuclear antigen show that in the control group positive rate was low, on the con-trary the tumor group was high expression; The treatment group were interven by drug The positive expression rate between the two before.2.9L induced tumor arise in the brain of the rats. implant duct In the striatum and9L cell3days after, kill5rats of control group7days after, no tumor occurrence; there are10rats of tumour group,whice means experim-ental operating technical was succeed.of tum our group,the first exist23days and the last exist34day and9Of10died within8days. Average life span (after implating) was26.8days. implanted9L cell and get out the brain of rats after7days, observe that mass bulge and ongitudinal cerebral fissure offset midlin e.By staining with HE cells are pleomorph-ic consistent with a malignant glioma.ConclusionAs2O3has therapeutic obvious effect on malignant glioma.and to provide experimental date for glioma treatment.