| Objective To study the expression of CD20of Ki-67in different types of thymoma, as well as to investigate the correlation between the expression of the two factors and biological characteristics of thymoma from the WHO classification and Masaoka stage. And thus it provided assistance for the early diagnosis of thymoma, pathological diagnosis, myasthenia gravis combine and prognosis,including objective indicators for the diagnosis and treatment.Method Surgically resected and pathologically proven56cases of thymoma and12cases of tissue of normal thymus were collected,which were from Shanghai Yodak Cardio-Thoracic Hospital and Fudan Clinical Pathology Diagnosis Center.All the patients received neither radiocherapy nor chemocherapy before the surgery. Data were recorded,including WHO classification and Masaoka stage.Expression of CD20and Ki-67were detected by immunohistochemistry S-P method. All the results were statistically analyzed with SPSS13.0software package.Results1.The rate of CD20positive of lymphocytes was different in various thymoma types (CD20expression of Thymoma neoplastic epithelial cells was positive in type A and type AB,which was confirmed and deleted.And the CD20expression of Thymoma neoplastic epithelial cells in type B and typeC was negative).There was no CD20positive expression in12cases of tissue of normal thymus. The rate of CD20positive expression was67.86%(38/56) in56cases of thymoma. The expression of CD20was significantly different between thymoma and tissue of normal thymus(χ2=18.457, P<0.05). There were colse relations between CD20and WHO classification. The positive expression rate of type A/AB, B1/B2/B3and C (thymic carcinoma) were57.14%、86.21%、16.67%respectively, and the expression of CD20was significantly difference in among type A/AB, B1/B2/B3and C (χ2=12.791, P<0.05). The rate of positive expression Stage I and II (81.82%)was significantly higher than III and IV (47.83%)(χ2=7.180, P<0.05) in Masaoka stage. The positive expression rate of MG group(83.3%) was higher than the uncombine MG group56.25%,which was significan difference between the two groups (χ2=4.612, P<0.05). The expression of CD20was no relevance of MG stage, age (χ2=0.136, P>0.05)and gender (χ2=3.238,P>0.05).2.Compared to tissue of normal thymus(0/12),the rate of Ki-67positive expression was higher in thymoma cases(55.36%). the rate of positive expression was significant difference (χ2=12.208, P<0.05). The rate of Ki-67positive expression was showed an increasing trend in the WHO classifications. The positive expression rate of type A/AB, B1/B2/B3and C (thymic carcinoma) were28.57%、68.97%、83.33%respectively, and the expression of Ki-67was significantly difference in among type A/AB, B1/B2/B3and C (χ2=10.17,P<0.05). In Masaoka staging:the rate of positive expression Stage III and IV (86.96%) was significantly higher than I and II (33.33%), which difference was statistically significant (χ2=15.770, P<0.05). The expression of CD20was no relevance of MG (χ2=3.186, P>0.05), age (χ2=0.107, P>0.05)and gender (χ2=1.005, P>0.05).3.There were significant negative correlations between CD20and Ki-67in thymoma(rs=-0.618, P<0.05).Conclusions1.The expression of CD20in human thmic tumors is closely related to invasive thymoma,pathological type and Masaoka stage,which suggest that it maybe played a role in occurrence and development of thymoma tumor,meanwhile, the expression of CD20is related to combined with MG, but is unrelated to stage of MG.2.The over-expression of Ki-67in human thymic tumors is obviously associated with the differentiation degree of the tumor,which can be referred to as an important index reflecting the malignant degree and progress of tumor.3.The joint detection of both plays a significant importance in understanding the occurrence and development, the early diagnosis of thymoma,and also provides objective basis for prognosis of patients with thymoma. |
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