Effects Of Expression Of β-catenin And CyclinD1Proteins Induced By PDTC In MG63Cell

Objective:Alveolar bone is the base of the dental restoration after teeth loss. Alveolar boneundergo progressive reconstruction after teeth missing, and the resorption rate is fastin the first three months. Bone metabolism is controlled by the balance of osteoblastsand osteoclasts, once the balance is disturbed, bone formation and resorption will beout of control. Osteoblastic differentiation and the progression of bone formation arecontrolled by several signaling pathways, mainly including ERK，MAPK，NF-κB，RANKL/OPG， BMP-2/Smads， Wnt/β-catenin pathways. In recent years theWnt/β-catenin and NF-κB pathways are essential hot in bone metabolism. If there is“interaction” between osteoblasts has not been confirmed.Method:Different concentration (0,100,200,400μ mol/L) PDTC were added to MG63cells then were incubated in CO2thermotank.(1) Cell proliferation at different points(12h,24h,48h,96h) were detected by MTT test.(2) RT-PCR assay was applied totest the expression of β-catenin at several point(12h,24h,48h);(3) Western-blot wasused to detect the expression of β-catenin and CyclinD1.Results:1. In the MTT assay, MG63cells were inhibited by different concentrations(100、200、400μmol/L). The inhibitional effect was increased with the increase ofconcentration and time, in a time and concentration manner.2. The results of RT-PCR showed PDTC(100、200、400μmol/L) had significanteffect on the expression of β-catenin mRNA in a dose and time-dependentmanner,and had statistical significance(p<0.01).3. As is showen in Western-blot, the expression of β-catenin present obvious increase with PDTC concentration increasing，and had statistical significance except400μmol/L group.The change in24hours to48hours had no statistical significance at400μmol/L group. CyclinD1proteins present obvious increasing trend in the earlytime, then had a decreased trend from24hours. The same as β-catenin, the CyclinD1also had statistical significance except400μmol/L group.The change in24hours to48hours had no statistical significance at400μmol/L group.Conclusion：1. The inhibition effect of NF-κB inhibitor pyrrolidine dithiocarboxylicacid(PDTC) on MG63cells proliferation led to increased expression of CyclinD1.2. The “interaction” between NF-κB and Wnt pathways in MG63cells involvedin the expression of CyclinD1.3. NF-κB inhibitor pyrrolidine dithiocarbamate regulating the expression of piv-otal protein of Wnt/β-catenin pathway, which increased β-catenin levels in MG63cells.