Diagnostic Value Of DNA Aneuploidy And Lunx MRNA Analysis In Pleural Fluid Of Non-Small Cell Lung Cancer Patients

Objective: Non-small cell lung cancer (NSCLC) which account for80-85%of the total number of lung cancer is often accompanied by pleuralfluid in advanced stage. Pleural fluid may also be the first symptom ofthe part of patients in some NSCLC patients which can not even find theprimary tumor. The sensitivity of the conventional cytological diagnosisbased on pathology is very low, due to the small number of cells andnon-typical cell morphology in pleural fluid. Establishing a set ofscientific and effective diagnostic method would be an effective methodto improve the positive rate of the diagnosis of lung cancer and thesurvival rate of patients with NSCLC. The subject is to evaluate thediagnostic values of DNA aneuploidy and Lunx mRNA expression of pleuralfluid of patients with NSCLC. Methods: Pleural fluid samples werecollected from the Jilin Province People’s Hospital．There were46casesof non-small cell lung cancer pleural fluid,22cases of non-lung tumorand15cases of benign lung diseases as controls. Fresh pleural fluidsamples were collected by anticoagulation tube with heparin. Record theDNA heteroploid value and DNA Index of pleural fluid samples byflowcytometry. Based on the construction of a standard curve for Lunx mRNAby TaqMan probes, Lunx mRNA expression of pleural fluid samples weredetected. Results: There were significant differences in DI, SPF%and PI%between pleural fluid of patients with NSCLC groups and benign disease(p<0.05), when the tumor excluding lung tissue have no difference withthe NSCLC patients in DI. The positive rate of Lunx mRNA for NSCLC was 91.3%(42/46) and had a statistical significance with the other twogroups(p<0.05).25in46patients with NSCLC detected double positiveresults and showed a statistical significance with other two groups(p<0.05). Conclusion: DI can be used as a sensitive detection indicatorreflecting the presence of malignant cells in the pleural fluid. Lunx mRNAexpression detected by FQ-RT-PCR have a high detection specificity. Jointdetection of DNA-aneuploidy and Lunx mRNA could improve the sensitivityand specificity of pleural fluid. They may provide high diagnostic valuesin NSCLC patients and might benefit further clinical procedures.