Protection Effect Of Hypoxia/Reoxygenation Injury In Primary Cultured Cardiac Myocytes And The Mechanism Study Of SheXiang BaoXin Pill

She Xiang Bao Xin Pill is a classic formula for treating angina, pleura and myocardial infarction resulting from ischemia in cardiac muscle. Nowadays, It’s widely used as a specific medicine for coronary heart disease in Chinese clinic practice. Although many achievements about pharmacological researches have been got on the level of whole animal body and organs and tissues, It’s still far away to explain the mechanism of this formula. In order to elucidate the molecular mechanism, target and pathway of SBP, we intend to investigate the protective effect of SBP and its primary bioactive compounds from the damage caused by hypoxia and reoxygenation on cardiomyocytes, based on the existing work. And adopt the technology of genomics to explain the the mechanism of SBP in therapying coronary heart disease.To study the protective effect on cardiac myocytes in the hypoxia/reoxygenation (H/R) injury of She xiang Bao xin Pill (SBP), hypoxia-reoxygenation(H/R) model was build using primary cultured rat cardiac myocytes, and MTT assay was used to evaluate cell viability of SBP on cardiac myocytes. The data demonstrated that SBP could protect cardiac myocytes in the hypoxia/reoxygenation (H/R) injury wiht the dosage of30,50,100and150μg/ml. Moreover, to study the active compounds in SBP, cardiac myocytes were treated with20compounds, and MTT assay was used to evaluate cell viability. The result showed that ginsenosideRb1, ginsenosidesRb2, bufalin and muscone could protect cardiac myocytes in the hypoxia/reoxygenation (H/R) injury wiht the dosage of10μM. In order to further study the protection mechanism of SBP on cardiac myocytes, Gene chip was used to study the difference gene expression profiles durig Rbl treated group, the results showed that there were38different genes when H/R group compared with normal group, including16up-regulation genes and22down-regulation genes;8different genes when Rb1treated group compared with H/R group, including6up-regulation genes and2down-regulation genes And Rb1regulated five genes, they were Sec11c, Ankrd11, Zfp70911, Scol and ND6. Through bioinformatics analysis, we attributed these genes to relative pathways. Through comparison, we found that there were mainly several pathways which might be the right way through which Rb1regulated to protect cardiac myocytes in H/R injury:Rb1might have reversed the low expression of ND6induced by H/R injury through oxidative phosphorylation or Parkinson’s disease pathways; Rb1might have reversed the low expression of Sec11c induced by H/R injury through Protein export pathways; Or reversed the low expression of Ankrd11、Zfp70911and Scol. However, these speculation were only according to the superficial phenomenon, so we have to do more to verify the inference.This work showed that SBP could protect cardiac myocytes in the hypoxia/reoxygenation (H/R) injury, and the target gene was initially studied. These have a certain meanings in clarifying pharmacodynamic substance foundation of SBP, and provide an idea in discovering the active compound in traditional Chinese Medicine.