Bushen Decoction On Ovariectomized Rats Lumbar Disc Tissue Interleukin-1Experimental Study Of Intervention

Objeetive:The purpose of this study is to observation by the kidney and blood soup fed ovariectomized rats lumbar disc tissue morphology and IL-1, nucleus pulposus notochord cell levels and expression evaluation of experimental efficacy of the kidney and promoting blood soup, explore kidney and activating bloodthe soup part of the prevention and treatment of lumbar disc degeneration molecular biology mechanism, and to delay or prevent lumbar intervertebral disc natural degeneration objective evaluation, compared with saline, confirmed Bu shen Decoction on prevention and treatment of lumbar disc degeneration.Method:8-month-old,100healthy female SD rats, weighing350-400g, provided by the Experimental Animal Center of Hunan Academy of Traditional Chinese Medicine and feeding.100rats were randomly divided into three groups, A control group of20B sham group20, C60surgery group.60C operation group, were randomly divided into C1castrated group C2castration drench group of C3positive drug control group,20each. Operation group castrated rat model manufactured in accordance with the Abstract ovarian surgery, sham operation group were taken next to both ovaries and ovarian adipose tissue of the same size. Modeling the beginning of the week gavage drugs, experimental group received kidney and promoting blood soup orally, the positive control group to be estradiolvalerate gavage, model group, sham operation group and blank control group were given an equal volume of saline, once a day. In perfusion after6weeks and12weeks after each group of10rats were sacrificed, remove the rat lumbar part, remove soft tissue around the vertebral body through the L5/6intervertebral remove the disc local tissue, immunohistochemistry the method observed lumbar disc tissue morphological changes of lumbar intervertebral disc nucleus pulposus number of notochord cells and interleukin-1positive expression changes, and compared with each other.Result:1.Pathology results:throughout the course of treatment, the control group, sham operation group notochord cells gradually reduced, was not statistically significant (P>0.05), uneven distribution of nucleus pulposus cells of the model group after6and12weeks of treatmentnotochord cells significantly reduced sham group compared with the control group, the number of notochord cells was significantly reduced, and with the time difference was significant (P<0.05), kidney blood circulation soup group with estradiol valerate group and treatment6after12weeks, the number of notochord cells less than the model group, the difference was significant (P<0.05), traditional Chinese medicine group and the positive drug group in the notochord cells after6and12weeks of treatment, there was no significant difference (P>0.05). 2.Immunohistochemical results:blank group after6weeks of treatment, sham operation group intervertebral disc tissue IL-1almost no expression saline group showed strong staining reaction kidney blood circulation soup group and estradiol valerate group staining reaction,protein expression in comparison with normal model group than in the control group, sham operation group, there was significantly higher (P<0.05), no significant difference (P>0.05), kidney and blood soup, kidney blood circulation soup group with estradiol valerate group between the twogroup, estradiol valerate group and control group, there was significant difference (P<0.05).Conclusion:1,castration can accelerate the degenerative disc;2, Bushenhuoxue soup and estrogen on lumbar disc degeneration in ovariectomized rats significantly delayed effect, and can reduce plasma and intervertebral disc through local organization such as inflammatory cytokines IL-1levels and regulation of nucleus pulposus notochord cells to promote bone cell production, and delay disc degeneration.