Effects Of50Hz Magnetic Field On Some Hemoto-biochemical Parameters And Organ Histomorphology In Rats

OBJECTIVE:With the development of economy and the coming of the information era, various types of electronic products and equipments are widely used at home and in workplace, which causes growing concerns about possible health effects of50Hz Magnetic exposure. With the common techniques in the clinical laboratory medicine and histopathology, We systematically observed the effects of50Hz magnetic field exposure on some hemoto-biochemical parameters and organ histomorphology in rats, and aimed to further study the biological effects of50Hz magnetic field, and tried to find blood serum biomarkers which could provide clues for the occupational health surveillance and health service administration (e.g. determine of special health inspection items, administration of health surveillance records, analysis of health status, evaluation of preventive measure, revise of sanitary standards) related to50Hz magnetic field exposure.METHODS:SD male rats were randomly divided into four groups, one control group and three irradiated groups, which were exposured to0μT,20μT,100μT,500μT50Hz magnetic field respectively for2hours per day in a period of4weeks. The body weight was recorded weekly. At the endpoint of exposure, peripheral blood, heart, liver, kidney, testes were collected. Then Parameters associated with liver and kidney function, blood lipids, myocardial enzymes, testosterone levels and peripheral blood cell number were determined. In addition, the weight of heart, liver, kidney and testes were detected and the organ index was calculated. and pathological changes of various organs and tissues were detected by light microscopy.RESULTS:There was no significant difference in body weight during50Hz magnetic field exposure.20μT and100μT50Hz magnetic field exposure have no obvious effect on the serum liver function, kidney function, blood lipids, myocardial enzymes, testosterone levels, each organ index and the number of peripheral blood cells. After500μT50Hz magnetic field exposure, the levels of creatinine and testosterone and the number of peripheral blood leukocyte were significantly higher than that of other groups (P<0.05), however, its total cholesterol levels in serum decreased significantly (P<0.05). There was no significant difference of the organ index among heart, liver, kidney and testicular tissue (P>0.05). The levels of serum creatinine, total cholesterol, testosterone and the number of peripheral blood leukocyte were significantly correlated with50Hz magnetic strength respectively (p<0.05). The myocardial interstitial vascular dilatation and congestion were observed. In addition, the dilatation and congestion of hepatic central vein sinusoidal and the dema of surrounding liver cells were also observed. In kidney, cloudy swelling of renal tubular epithelial cells and dilatation and congestion stromal vascular were noted. Besides, the focal congestion and edema were visualized in testicular interstitial tissue. In addition, the severity of the above morphologic change is positively correlated with the dose of exposure.CONCLUSIONS:The exposure to50Hz magnetic field has no significant effect on the growth and development of male rats, and can result in increase of vasopermeability in organs and tissues in rats. The exposure to50Hz magnetic field of500μT is able to cause changes of the kidney function, testicular function, lipid metabolism, and the increase of peripheral blood leukocyte in rats. The levels of serum creatinine, total cholesterol, testosterone and the number of peripheral blood leukocyte may be the potential serum biomarkers related with50Hz magnetic field exposure, and remains to be intensely researched.