| ObjectiveSelect the nerve damage of the distal nerve fragments of different lengthsin series chitosan-collagen-CNTF chamber of the rabbit sciatic nerveregeneration.MethodsForty Japanese white rabbits were randomized in four groups of10, A:6mm nerve fragment in series chamber group; B:8mm nerve fragment inseries chamber group; C:10mm nerve fragment in series chamber group; D:nerve autograft group. The experimental group in the big white rabbit left thesciatic nerve place cause about12mm of nerve defects, and in each groupdamage nerve far end take4mm respectively,6mm,8mm, nerve segments,regeneration chamber was transplantation after series back to nerve defect,the control group in the big white rabbit left the sciatic nerve place about22mm caused nerve defects in situ after transplantation.General observation atdifferent times after surgery, and after16weeks in electrophysiology,gastrocnemius wet weight, histology, electron microscopy and image analysisevaluation of nerve regeneration.Result16weeks after B, C, D groups in nerve conduction velocity, gastrocnemiuswet weight percentage of the difference was not statistically significant, arebetter than group A, and the difference was statistically significant (P<0.05).16 weeks after operation the number of regenerating nerve fibers, diameter andthickness of the myelin sheath compared four groups in the proximal differencewas not statistically significant, in the remote office B, C, D between the twogroups no significant difference, are excellent in group A, and the differencewas statistically significant (P<0.05).ConclusionThe regeneration chamber which selected from autologous nerve in injurydistal nerve has obvious effect in repairing peripheral nerve defects,6mm and8mm group is better than4mm group. Besides, the results are similar to theautologous nerve graft. But the6mm group has the advantage of savingnerves, which is the most suitable autologous nerve fragment length. |
PDF Full Text Request