Effects Of Repairing Function And Expression Of Growth Associated Protein-43by Silence Ephrin-B3after The Spinal Cord Injury In Rats

ObjectiveObservation the effects of spinal cord function through si-Ephrin-B3afterinjury in rats, providing the feasible experimental basis for clinical treatment.MethodsUse of SiRNA targeted the jamming technology, constructing three groupsof the gene for Ephrin-B3SiRNA interference plasmid (used in three groups ofthe experimental group A/B/C group) and one group A sequence SiRNAinterference plasmid (control group that is used in group D), in vitro cloning andextracting the plasmid packing slow virus particles. Half a crosscut legal systemfor adult rat spinal cord damage model, SiRNA slow virus particles into thespinal cord injury in rats. After injection respectively in1w/2w/4w/8w, Usesthe BBB evaluation method is in each period of experimental animals limbfunction recovery; The spinal cord tissues extracted with RealTime-PCR methodto detect gene expression in Ephrin-B3after injury; By immunohistochemicalmethod observe the expression of Gap-43changes.ResultsNormal rats relatively low Ephrin-B3expression, detection1w~8w, Dgroup visible Ephrin-B3into clear ascendant trend, and A, B and C three groupsof SiRNA all play A Ephrin-B3silence effects, particularly group B; after1~4w,B rats, BBB is rising in the ratings trends, are higher than the other three groups,D group was significantly lower than the other three groups,4w group afterrestoring effect of the platform; In immuohistochemisty the Gap-43was found significantly expressed in group B than other groups, the high expression trendafter transplantation from1w，4～8w have come down.ConclusionsSilence Ephrin-B3will promote rat spinal cord injury early function repair,and raised the Gap-43expression.